Purification of Secretory IgA from Bovine Colostrum
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概要
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A fraction containing IgA (IgA-rich fraction) was prepared from bovine colostrum by anion exchange chromatography using DEAE-Sephadex A-50 and gel filtration on Sephadex G-200. A large amount of IgG1-dimer was found in this fraction, which could not be separated from IgA by repeated gel filtration. The Fc fragment of bovine colostral IgG (IgG-Fc) was prepared from papain digestion mixtures. IgG-Fc was found to be heterogeneous on DEAE-celluiose column chromatography. Two IgG-Fc fractions were obtained, but no antigenic difference was found between them. Anti-IgG-Fc antibodies raised in rabbits by injection of these Fc preparations reacted only with IgG1 and IgG2. An immunoadsorbent (anti-IgG-Fc-Sepharose) was prepared by coupling these anti-IgG-Fc antibodies to CNBr-activated Sepharose 4B. IgA was purified from the IgA-rich fraction by affinity chromatography on anti-IgG-Fc-Sepharose adsorbent. IgG1-dimer was effectively removed by this treatment. The purified sample gave only one precipitin arc characteristic of IgA on immunoelectrophoresis with multiple anti-bovine colostral whey antiserum. A small amount of IgA was found to be adsorbed to the affinity column nonspecifically. When a rabbit was immunized with the purified IgA, besides anti-IgA antibodies, antibodies against the secretory component (SC) were found in the antiserum. This finding leads us to expect that the purified IgA is secretory IgA containing SC.
- 社団法人 日本農芸化学会の論文
著者
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Kuzuya Yasuo
Department Of Food Science Faculty Of Agriculture Gifu University
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Kanamaru Yoshihiro
Department Of Applied Life Science Faculty Of Applied Biological Sciences Gifu University
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KANAMARU Yoshihiro
Department of Poultry and Animal Sciences, Faculty of Agriculture, Gifu University
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TANAHASHI Tamotsu
Department of Poultry and Animal Sciences, Faculty of Agriculture, Gifu University
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Tanahashi Tamotsu
Department of Cardiology, Suzuka National Hospital, Japan
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KUZUYA Yasuo
Department of Poultry and Animal Sciences, Faculty of Agriculture, Gifu University
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