Involvement of Both the N-Glycan-relevant and N-Glycan-irrelevant Structural Elements in the Recognition of Human Milk Lactoferrin by the 1CF11 Monoclonal Antibody
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概要
- 論文の詳細を見る
Monoclonal antibody 1CF11 has been suggested to specifically recognize a certain carbohydrate epitope shared by glycoproteins in human external secretions. We examined the effect of cleaving the polypeptide backbone and removing N-linked oligosaccharides on the reactivity with 1CF11 of human milk lactoferrin (hLf) to elucidate the structural features of the 1CF11 epitope. We reveal by treating hLF with trypsin and/or N-glycosidase that both the N-glycan-relevant and N-glycan-irrelevant structural elements were involved in the recognition of hLf by 1CF11.
- 社団法人 日本農芸化学会の論文
- 2010-10-23
著者
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金丸 美敬
Dep. Of Applied Life Sci. Gifu Univ.
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Kanamaru Yoshihiro
Department Of Food Science Faculty Of Agriculture Gifu University
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Kanamaru Yoshihiro
Department Of Food Science Faculty Of Applied Biological Science Gifu University
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Kanamaru Yoshihiro
Department Of Applied Life Science Faculty Of Applied Biological Sciences Gifu University
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Kanamaru Yoshihiro
Faculty Of Applied Biological Sciences Gifu University
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YABE Tomio
Department of Applied Life Science, Gifu University
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Fukaya Shinichi
The United Graduate School Of Agricultural Science Gifu University
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SHINODA Chisato
Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University
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Shinoda Chisato
Department Of Applied Life Science Faculty Of Applied Biological Sciences Gifu University
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Yabe Tomio
Department Of Applied Life Science Gifu University
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Yabe Tomio
United Graduate School of Agricultural Science, Gifu University
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