数種の in vitro アッセイを用いた我国沿岸海水および河川水中のエストロゲン様物質の測定

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To determine the level of estrogenic substances in an aquatic environment, four types of in vitro assay, namely, E-Screen, Ishikawa cell-ALP, YES and ELISA, and chemical analysis using GC/MS were carried out. Water samples were collected from coastal areas and rivers in Japan. Estrogenic substances were extracted by solid-phase extraction using a Sep-pak C18 cartridge. Higher levels of estrogenic substances were observed at sites close to sewage treatment plants as measured by in vitro assays. The 17β-estradiol equivalent concentration (E2 equiv.) determined by E-screen tended to be higher than those of the other assays, whereas YES showed a lower sensitivity than E-screen and Ishikawa cell ALP. According to the chemical analysis, the levels of estrone (E1) were higher than those of 17β-estradiol (E2). Although 17α-ethynylestradiol, nonylphenol, octylphenol and bisphenol A were detected in a few samples, their levels were low. E1 and E2 contributed to most parts of E2 equiv. in the water samples.
社団法人　日本水環境学会の論文
2004-12-10