Inhibitory Mechanism of Mizoribine on the Antibody Production of Mouse B Cells Stimulated with Lipopolysaccharide
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概要
- 論文の詳細を見る
It has been reported that the immunosuppressant mizoribine (MZR)inhibits T cell proliferation by depleting intracellular guanine nucleotides via competitive inhibition of inosine 5-monophosphate (IMP)dehydrogenase in the purine metabolism pathway. This study was performed to determine if the mechanism by which MZR suppresses the proliferation of mouse B cells and antibody production by these cells is dependent on the depletion of intracellular guanine nucleotides. Stimulation of purified splenic B cells of mice with lipopolysaccharide (LPS), a mitogen to B cells, increased both proliferation and antibody production. MZR suppressed both of these functions in a dose-dependent fashion. MZR also caused a decrease in the amount of intracellular guanosine 5-triphosphate (GTP). When the cultures were grown on plates containing guanosine plus 8-aminoguanosine, the amount of intracellular GTP, which had been reduced by MZR, was restored. Furthermore, the repletion of GTP pools restored both proliferation and antibody production almost to their previous levels. These results suggest that MZR suppresses antibody production and proliferation of B cells by acting directly on B cells. Furthermore, it is suggested that the inhibitory effect of MZR on antibody production, as well as on T cell proliferation, is dependent on the decrease in intracellular guanine nucleotide pools of mouse B cells.
- 社団法人 日本薬理学会の論文
- 1997-08-01
著者
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NAKAGAMI Keiji
Laboratory for Immunology, Institute for Life Science Research, Asahi Chemical Industry Co., Ltd.,
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Nakagami Keiji
Laboratory For Immunology Institute For Life Science Research Asahi Chemical Industry Co. Ltd.
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Shibata Hiroshi
Laboratory For Health Care Science Research Center Suntory Ltd.
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Itoh H
Kinki Univ. Higashi‐osaka Jpn
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KAMADA Hiroyuki
Laboratory for Immunology Institute for Life Science Research, Asahi Chemical Industry Co., Ltd.
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ITOH Hiromichi
Laboratory for Immunology Institute for Life Science Research, Asahi Chemical Industry Co., Ltd.
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KOSHIO Takehiro
Laboratory for Immunology Institute for Life Science Research, Asahi Chemical Industry Co., Ltd.
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HAYASHI Akira
Laboratory for Chemistry, Institute for Life Science Research, Asahi Chemical Industry Co., Ltd.
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Koshio Takehiro
Laboratory For Immunology Institute For Life Science Research Asahi Chemical Industry Co. Ltd.
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Kamada Hiroyuki
Laboratory For Immunology Institute For Life Science Research Asahi Chemical Industry Co. Ltd.
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Shibata Hiroshi
Laboratory For Immunology Institute For Life Science Research Asahi Chemical Industry Co. Ltd.
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Hayashi Akira
Laboratory For Chemistry Institute For Life Science Research Asahi Chemical Industry Co. Ltd.
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