Analytical Method for Routine Quantitative Abalysis of Plant Stanols in Stanol Ester Spread
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概要
- 論文の詳細を見る
Clinical studies on foods containing plant stanol ester repeatedly demonstrate 10-14% reduction in serum LDL-cholesterol. Since plant stanols are the agents responsible for the cholesterol-lowering activity of stanol ester, an analytical method was developed for quantitative and routine analysis of plant stanol content in spreads containing clinically-effective levels of plant stanol ester. Analysis of plant stanols in stanol ester spread is achieved using direct saponification in which stanol ester spread is saponified directly with ethanolic potassium hydroxide without prior separation of the lipid fraction. Plant stanols are then extracted with hexane and derivatized to trimethylsilyl ethers. Plant stanols are analyzed by Gas Chromatography and quantified against cholestanol as the Internal Standard. Analysis of sitostanol, campestanol and total stanol content in spreads supplemented with stanol ester was found to be linear (r2>0.999), accurate (99-102% recovery), precise (RSD<2.0%) and rugged between laboratories. These results demonstrate that the analytical methods for plant stanol detemination are validated for routine quantitative analysis of spreads containing plant stanol ester.
- 日本油化学会の論文
- 2001-03-01
著者
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Sugano Michihiro
Laboratory Of Food Chemistry Division Of Bioresource And Bioenvironmental Sciences Graduate School K
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Shapiro Kenneth
Mcneil Consumer Healthcare Research And Development Laboratories
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LI Li
McNeil Consumer Healthcare, Research and Development Laboratories
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SECOR Candyce
McNeil Consumer Healthcare, Research and Development Laboratories
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Secor Candyce
Mcneil Consumer Healthcare Research And Development Laboratories
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Li Li
Mcneil Consumer Healthcare Research And Development Laboratories
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