Different domains of Sgs1 are required for mitotic and meiotic functions
スポンサーリンク
概要
- 論文の詳細を見る
The SGS1 of Saccharomyces cerevisiae is a homologue for human Blooms syndrome, Werners syndrome, and Rothmund-Thomsons syndrome causative genes. Disruptants of SGS1 show high sensitivity to methyl methanesulfonate (MMS) and hydroxyurea, and hyper recombination phenotypes including interchromosomal homologous recombination in mitotic growth. In addition, sgs1 disruptants show poor sporulation and a reduced level of meiotic recombination as assayed by return-to-growth. We examined domains of Sgs1 required for mitotic and meiotic functions of Sgs1 by transfecting variously mutated SGS1 into sgs1 disruptants. The N-terminal 1-401 amino acid region was required for complementation of MMS sensitivity and suppression of hyper heteroallelic recombinations of sgs1 disruptants in mitotic growth and for complementation of poor sporulation and of reduced meiotic recombination. Although the N-terminal 1-125 amino acid region was absolutely required for the complementation of MMS sensitivity and suppression of hyper heteroallelic recombinations in mitotic growth, it was dispensable for the meiotic functions. In contrast, the highly acidic region (400-596 amino acid) was dispensable for the mitotic functions but a deletion of this region affected the meiotic functions. The C-terminal 1271-1350 amino acid region containing a HRDC (helicase and RNaseD C-terminal) domain was dispensable for the mitotic and meiotic functions. Although DNA helicase activity of Sgs1 was not required for Sgs1 to complement the meiotic functions, a deletion of helicase motifs III-IV (842-1046 amino acid) abolished the complementing activity of Sgs1, indicating that a structurally intact helicase domain is necessary for Sgs1 to fulfill its meiotic functions.
- 日本遺伝学会の論文
- 2000-12-01
著者
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OHNO Yasuo
Division of Pharmacology, National Institute of Health Science
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MIYAJIMA Atsuko
Division of Pharmacology, National Institute of Health Sciences
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Miyajima A
Division Of Pharmacology Biological Safety Research Center National Institute Of Health Sciences
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Miyajima Atsuko
東北大学 薬研究 遺伝子薬
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Miyajima Atsuko
Division Of Pharmacology Biological Safety Research Center National Institute Of Health Sciences
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SEKI Masayuki
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University
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UI Ayako
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University
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SATOH Yurie
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University
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ONODA Fumitoshi
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University
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ENOMOTO Takemi
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University
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Ui Ayako
Molecular Cell Biology Laboratory Graduate School Of Pharmaceutical Sciences Tohoku University
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Satoh Yurie
Molecular Cell Biology Laboratory Graduate School Of Pharmaceutical Sciences Tohoku University
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Seki Masayuki
Molecular Cell Biology Laboratory Graduate School Of Pharmaceutical Sciences Tohoku University
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Enomoto Takemi
Molecular Cell Biology Laboratory Graduate School Of Pharmaceutical Sciences Tohoku University
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Enomoto T
Tohoku Univ. Miyagi
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Onoda Fumitoshi
Molecular Cell Biology Laboratory Graduate School Of Pharmaceutical Sciences Tohoku University
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Enomoto T
Molecular Cell Biology Laboratory Graduate School Of Pharmaceutical Sciences Tohoku University
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Enomoto Takemi
Cell Biology Laboratory Graduate School Of Pharmaceutical Sciences Tohoku University
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Seki M
Nissin Kogyo Co. Ltd. Tobu Jpn
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Ohno Yasuo
Division Of Internal Medicine And Thoracic Oncology National Cancer Center Hospital
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Ohno Yasuo
Division Of Pharmacology Biological Safety Research Chenter National Institute Of Health Sciences
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