Sexing of postimplantation rat embryos in stored two-dimensional electrophoresis (2-DE) samples by polymerase chain reaction (PCR) of an Sry sequence
スポンサーリンク
概要
- 論文の詳細を見る
Proteomic analysis of developmental toxicity by two-dimensional electrophoresis (2-DE) may detect gender-related toxic effects in embryos without visible gender characteristics. In the present study, we explored sexing of rat embryo stored in frozen 2-DE samples by polymerase chain reaction (PCR) of a male-specific gene sequence, sex determining region Y (Sry). The embryo proper and yolk sac membrane at gestation day 11 from Wistar rats were used for stored embryonic 2-DE samples. The embryonic 2-DE samples were desalted and their total DNA was extracted. The Sry sequence in the extracted DNA was amplified by PCR and the product was analyzed by agarose gel electrophoresis. The embryos with the PCR product of Sry were determined as male, and those without the product were determined as female. It was concluded that stored embryonic 2-DE samples could be used for retrospective examination of gender-related effects in proteomic analysis of developmental toxicity.
- 日本トキシコロジー学会の論文
- 2009-12-01
著者
-
Miyajima Atsuko
Division Of Pharmacology National Institute Of Health Sciences
-
SUNOUCHI Momoko
Division of Pharmacology, National Institute of Health Sciences
-
Miyajima Atsuko
Division Of Pharmacology Biological Safety Research Center National Institute Of Health Sciences
-
NAKAZAWA Ken
Division of Pharmacology, National Institute of Health Sciences
-
Nakazawa Ken
Division Of Pharmacology National Institute Of Health Sciences
-
Mitsunaga Katsuyoshi
Graduate School of Pharmaceutical Sciences, Toho University
-
Yamakoshi Yoko
Department of Radiology, Department of Chemistry, University of Pennsylvania
-
Usami Makoto
Division of Pharmacology, National Institute of Health Sciences
-
Usami Makoto
Division Of Pharmacology National Institute Of Health Sciences
-
Yamakoshi Yoko
Department Of Nutrition And Food Science Ochanomizu University:(present Address) National Institute
-
Yamakoshi Yoko
Department Of Radiology Department Of Chemistry University Of Pennsylvania
-
Sunouchi Momoko
Division Of Pharmacology National Institute Of Health Sciences
-
Sunouchi Momoko
Division Of Pharmacol. Mutagen. Nihs
-
Mitsunaga Katsuyoshi
Graduate School Of Pharmaceutical Sciences Toho University
-
Mitsunaga Katsuyoshi
Graduate School Of Pharmaceutical Sciences Toho Univ.
-
Usami Makoto
Division Of Pathology National Institute Of Health Sciences
-
Usami Makoto
Division of Nutrition and Metabolism, Department of Biophysics, Kobe University Graduate School of Health Sciences
関連論文
- Novel Nonsynonymous Single Nucleotide Polymorphisms in the CYP2D6 Gene
- 21C-03-4 Effects of 2-Mercaptobenzimidazole (MBI) and its methyl derivatives MMBI on rat hepatic drug metabolizing enzymes.
- Sulfating-Activity and Stability of cDNA-Expressed Allozymes of Human Phenol Sulfotransferase, ST1A31 (^Arg) and ST1A32 (^His), Both of Which Exist in Japanese as Well as Caucasians^1
- "B-11 Effects of triazine herbicides on isolated and primary cultured hepatocytes of rat.
- THE EFFECT OF DIQUAT ON THE GAP JUNCTIONAL INTERCELLULAR COMMUNICATION BETWEEN RAT HEPATOCYTES IN PRIMARY CULTURE
- EFFECTS OF ORGANIC PHOSPHOROUS COMPOUNDS AND THEIR METABOLITES ON RAT HEPATOCYTES IN THE PRIMARY CULTURE
- Functional and physical interaction between Sgs1 and Top3 and Sgs1-independent function of Top3 in DNA recombination repair
- Different domains of Sgs1 are required for mitotic and meiotic functions
- Characterization of the Properties of a Human Homologue of Escherichia coli RecQ from Xeroderma Pigmentosum Group C and from HeLa Cells
- Modulation of Voltage-Gated Ca^ Current by 4-Hydroxynonenal in Dentate Granule Cells(Highlighted paper setected by Editor-in-cfiief)(Pharmacology)
- Sexing of postimplantation rat embryos in stored two-dimensional electrophoresis (2-DE) samples by polymerase chain reaction (PCR) of an Sry sequence
- STUDY ON THE SPERM MOTILITY, COUNT OR MORPHOLOGICAL CHANGE AND MALE FERTILITY DISTURBANCE INDUCED BY CHEMICALS
- "C-5 Validation of alternatives to eye irritation tests for cosmetic ingredients (Results of the first, second, and third validation).
- Effect of Estradiol and Nonyiphenol on mRNA Expression of Estrogen Receptors α and β, and Cytochrome P450 Aromatase in the Gonad of Chicken Embryos
- Suppressive Effect of p-Nonylphenol on Male-Specific mRNA Expression in the Embryonic Gonad of Chickens
- Effect of Estradiol and Nonylphenol on mRNA Levels of VitellogeninII in the Liver of Chicken Embryos
- CONFIRMATION STUDY, USING NITROBENZENE, OF THE COMBINED REPEAT DOSE AND REPRODUCTIVE/DEVELOPMENTAL TOXICITY TEST PROTOCOL PROPOSED BY THE ORGANIZATION FOR ECONOMIC COOPERATION AND DEVELOPMENT (OECD)
- A-1 MECHANISM UNDERLING NEUROTOXICITY OF CHLORDECONE : AN ELECTROPHYSIOLOGICAL APPROACH USING PC12 PHEOCHROMOCYTOMA CELLS
- Macrocarpals, Antibacterial Compounds from Eucalyptus, Inhibit Aldose Reductase
- EFFECTS ON FETAL GROWTH OF REPEATED BLOOD COLLECTION FOR TOXICOKINETICS FROM PREGNANT RATS
- COMPARATIVE TOXICOKINETIC STUDY OF RUBBER ANTIOXIDANTS, 2-MERCAPTOBENZIMIDAZOLE AND 2-MERCAPTOMETHYLBENZIMIDAZOLE, BY SINGLE ORAL ADMINISTRATION IN RATS
- Effects of Tamoxifen on L-Glutamate Transporters of Astrocytes
- Developmental toxicity of indium : Embryotoxicity and teratogenicity in experimental animals
- 219 EFFECTS OF 2-MONOCHLORODIBENZOFURAN AND 3-MONOCHLORODIBENZOFURAN ON THE P450-MEDIATED DRUG METABOLIZING ENZYME ACTIVITIES OF PRIMARY CULTURED RAT HEPATOCYTES : COMPARISION WITH THE EFFECTS OF DIOXINS AND METHLCHOLANTHRENE
- STUDIES ON THE ESTABLISHMENT OF APPROPRIATE SPERMATOGENIC ENDPOINTS FOR MALE FERTILITY DISTURBANCE IN RODENT INDUCED BY DRUGS AND CHEMICALS I. NITROBENZENE
- STUDY OF EXAMINATION METHODS FOR ASSESSING MALE FERTILITY DISTURBANCES 2.THE SPERM EVALUATION USING ADRIAMYCIN
- EFFECTS OF VARIOUS INDUCERS ON THE ACTIVITIES OF CYTOCHROME P-450-MIXED FUNCTION OXIDASES AND AFLATOXIN B_1 ACTIVATION IN MICROSOMES OF HAMSTER LIVERS
- IN VITRO NEUROTOXICITY ASSAY SYSTEM BY NEURONAL CLONES (1) : EFFECTS OF CHLORDANE ON ELECTROPHYSIOLOGICAL PROPERTIES OF HYBRID NEURONAL CLONE NG 108-15
- A-15 IN VITRO TERATOGENICITY TESTING USING THE RAT EMBRYO CULTURE SYSTEM : (3) EFFECTS OF CYCLOPHOSPHAMIDE ON RAT EMBRYONIC DEVELOPMENT IN VITRO AND IN VIVO
- A-6 EFFECT OF BIS (TRIBUTYLTIN) PHTHALATE ON THE FETAL DEVELOPMENT
- Two Non-steroidal Anti-inflammatory Drugs, Niflumic Acid and Diclofenac, Inhibit the Human Glutamate Transporter EAAT1 Through Different Mechanisms
- Block by Phytoestrogens of Recombinant Human Neuronal Nicotinic Receptors
- Isolation and Characterization of Macrocarpals B-G Antibacterial Compounds from Eucalyptus macrocarpa
- Gender Differences of Dietary Self-Management Behavior Affecting Control Indices in Type II Diabetes
- Effect of Ultrasound Irradiation on α-SMA and TGF-β1 Expression in Human Dermal Fibroblasts
- Effects of Tamoxifen on L-Glutamate Transporters of Astrocytes
- Inhibition of Ion Channels by Hirsutine in Rat Pheochromocytoma Cells.
- Non-Competitive Antagonism by Hirsuteine of Nicotinic Receptor-Mediated Dopamine Release from Rat Pheochromocytoma Cells.
- Sulfating-Activity and Stability of cDNA-Expressed Allozymes of Human Phenol Sulfotransferase, ST1A3*1 (213Arg) and ST1A3*2 (213His), Both of Which Exist in Japanese as Well as Caucasians.