Effect of Ultrasound Irradiation on α-SMA and TGF-β1 Expression in Human Dermal Fibroblasts

概要

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Ultrasound therapy is used to promote pressure ulcer healing as an adjunctivetherapy. However, the efficacy and the scientific basis of this treatment are unclear. Weinvestigated the effect of ultrasound irradiation on alpha-smooth muscle actin (α-SMA)and transforming growth factor-beta1 (TGF-β1) expression in human dermalfibroblasts. These are important factors for acceleration of wound closure. We usedpulsed ultrasound of 0, 0.1, 0.5, and 1.0 W/cm2. TGF-β1 and α-SMA mRNA wasmeasured by quantitative real-time polymerase chain reaction, α-SMA protein wasexamined by western blot, and localization of α-SMA was evaluated byimmunofluorescence staining. Expression of α-SMA and TGF-β1 mRNA was increasedat 24 h but not at 48 h after ultrasound irradiation. There were significant differencesbetween controls of 0 W/cm2 and 0.1 W/cm2 with a 1.34 ± 0.26 fold increase in α-SMA(P < 0.05) and a 1.78 ± 0.57 fold increase in TGF-β1 (P < 0.05). Protein levels of α-SMAwere also increased and detected in ultrasound irradiated fibroblasts at 24 h.Ultrasound irradiation promotes α-SMA expression in human dermal fibroblasts andthis suggests the biological mechanism of ultrasound efficacy on chronic woundtreatment.
神戸大学の論文
2010-00-00