Cellular Fatty Acid Composition of Stratified Squamous Epithelia After Transplantation of Ex Vivo Produced Oral Mucosa Equivalent
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概要
- 論文の詳細を見る
Purpose: We have fabricated, for clinical application, artificial oral mucosa thattotally excludes both heterogenic protein interaction and xenotransplantation. Thepurpose of this study is to compare the fatty acid composition of cell membranephospholipids related to post-transplantation epithelial regeneration.Materials and Methods: Cultured keratinocytes, keratinocytes at 2, 3, 4, and 9weeks after transplantation, and normal oral keratinocytes were compared by gaschromatography for the composition of 23 fatty acids. The relation between thecomposition of cell membrane fatty acids, and the glucose metabolism wasimmunohistochemically analyzed.Results: 1. Even after transplantation, cultured keratinocytes retained the sameratio of palmitic acid as that of normal oral keratinocytes. 2. Essential fatty acidsdecreased markedly in cultured keratinocyte membranes to the same composition asthat of normal oral mucosa 2 weeks after transplantation. 3. The percent compositionof palmitoleic acid in cultured keratinocytes was significantly higher than that inpost-transplanted keratinocytes; it decreased 2 weeks after artificial mucosatransplantation, but became similar to that in 3 weeks thereafter. 4. The entirepopulation of stratified keratinocytes in EVPOME before transplantation expressedGLUT-1 protein.Conclusion: Our findings suggest that post-artificial mucosa epithelialisation allowskeratinocytes to proliferate while consuming palmitic acid, and then diet-providedessential fatty acids induce the keratinocytes to differentiate. Complete clinicalepithelialisation of the transplant wound requires 4 weeks; however, within 3 weeks oftransplantation, cultured cells of a specific metabolic mechanism change into or arereplaced by keratinocytes of a normal metabolic mechanism similar to that ofsurrounding tissue.
- 神戸大学の論文
- 2010-00-00
著者
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Yokoo Satoshi
Department Of Oral And Maxillofacial Surgery Kobe University School Of Medicine
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Terashi Hiroto
Department Of Dermatology Oita Medical University
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Komori Takahide
Department Of Endodontics Showa University School Of Dentistry
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YOKOO Satoshi
Department of Stomatology and Oral Surgery, Gunma University Graduate School of Medicine
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Komori Takahide
Department of Oral and Maxillofacial Surgery Kobe University Graduate School ofMedicine
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Moriyama Kentaro
Department of Oral and Maxillofacial Surgery Kobe University Graduate School ofMedicine
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