A New in vitro Invasion Model for Oral Cancer Using an Acellular Allogenic Dermal Matrix (Alloderm) : The Relationship among in vitro Invasion Activity, in vivo Invasion and Metastasis
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概要
- 論文の詳細を見る
The purpose of this study was to develop a new in vitro model of cancer invasion using a human dermal equivalent, AlloDerm®. A squamous cell carcinoma line (HSC-2, HSC-3 and OSC20) and a malignant melanoma line (G361) were cultured on AlloDerm® and evaluated histologically to examine destruction of the basement membrane and invasion into the dermis of AlloDerm®. HSC-3 and G361 cells invaded through the basement membrane of AlloDerm® at 21-28 days after seeding, while HSC-2 and OSC20 cells did not. Next, these cells were transplanted into the tongues of nude mice, and the degree of local invasiveness was examined histologically. HSC-3 and G361 cells invaded diffusely to the surrounding tongue tissue, although HSC-2 and OSC20 showed only expansive growth. Further, these cells were transplanted subcutaneously in nude mice to study metastatic activity. Regional and distant metastases were rarely observed after transplantation of HSC-2 and OSC20 cells. On the other hand, HSC-3 and G361 cells frequently metastasized. These findings show a close relationship among in vitro invasiveness on AlloDerm®, in vivo invasiveness, and metastatic activity. This experimental model using AlloDerm® is a potentially new in vitro model of cancer invasion.
- 神戸大学の論文
- 2011-00-00
著者
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Shigeta Takashi
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine
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Shigeta Takashi
Department Of Mechanical Engineering And Materials Science Faculty Of Engineering Kumamoto Universit
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Takahashi Hidenori
Department Of Applied Biological Sciences Faculty Of Science And Technology Tokyo University Of Scie
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Komori Takahide
Department Of Endodontics Showa University School Of Dentistry
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Umeda Masahiro
Department of Clinical Oral Oncology, Nagasaki University Graduate School of Biomedical Sciences
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Komori Takehide
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine
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