The Long-term Effects of Red Light-emitting Diode Irradiation on the Proliferation and Differentiation of Osteoblast-like MC3T3-E1 Cells
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概要
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Low level laser therapy (LLLT) affects various biological processes, and it is said that the non-coherent light of the light-emitting diode (LED) has a similar action. The purpose of this study was to examine the effects of LED light on the proliferation and differentiation of osteoblasts-like MC3T3-E1 cells cultured in osteogenic differentiation medium (ODM) over the long term. Cells were irradiated with red LED light of 630 nm at three doses; 0.5J/cm2, 1.5J/cm2 or 3.0J/cm2 for the cell proliferation activity assay, and at 0.5J/cm2 for the osteogenic differentiation activity assay. The former activity was checked by counting the number of viable cells using Trypan blue dye. The latter activity was evaluated by alkaline phosphatase (ALP) staining and examining the mRNA expression of the osteopontin (OPN) gene using real-time quantitative PCR. The number of viable MC3T3-E1 cells showed a tendency to increase after the irradiation at all three energy densities in comparison with a non-irradiation group (control group). In particular, there was a remarkable 3.34-fold increase in the group irradiated with 3.0J/cm2 on day 7 after starting the culture. On culture day 15, there was a tendency for the red LED irradiation group (0.5 J/cm2) to exhibit more staining for ALP than the control group, and the expression of OPN was significantly higher in the irradiation group on culture day 16. In conclusion, low level red LED light can enhance MC3T3-E1 cell proliferation and osteogenic differentiation when the cells are cultured for a relatively long time.
- 神戸大学の論文
著者
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Kimoto Akira
Department Of Electrical And Electronic Engineering Faculty Of Science And Engineering Saga Universi
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Komori Takahide
Department Of Endodontics Showa University School Of Dentistry
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Suzuki Hiroaki
Department Of Advanced Materials Science University Of Tokyo
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Matsumoto Kousuke
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine
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Asai Tomoko
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine
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Kitayama Midori
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine
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Shigeoka Manabu
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine
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