Inhibition of Ion Channels by Hirsutine in Rat Pheochromocytoma Cells.
スポンサーリンク
概要
- 論文の詳細を見る
Effects of hirsutine, an alkaloid that produces a potent ganglion blocking effect, were investigated using rat pheochromocytoma PC12 cells. Hirsutine (1 to 10 μM) suppressed dopamine-release evoked by 100 μM nicotine. In voltage-clamped cells, hirsutine (1 to 10 μM) inhibited the inward current activated by 100 μM nicotine. Hirsutine was equipotent to hexamethonium in blocking the nicotine-activated current. The voltage-dependency of the nicotine activated current was not modified by hirsutine. Effects of hirustine on other ion channels were tested to determire its selectivity. Inward currents mediated through ATP-activated channels were scarcely affected by hirsutine (up to 100 μM). However, hirustine (10 μM) inhibited Ba currents passing through Ca channels and K currents activated by depolarizing voltage steps. The results suggest that hirsutine potently blocks nicotinic receptor-channels, but hirsutine also inhibits voltage-gated Ca and K channels. Roles of the inhibition of these channels in the pharmacological effects of hirsutine were discussed.
- 公益社団法人 日本薬理学会の論文
著者
-
Nakazawa Ken
Division Of Pharmacology National Institute Of Health Sciences
-
INOUE Kazuhide
Division of Biosignaling, National Institute of Health Sciences
-
Takanaka Akira
Division Of Pharmacology Bsrc National Institute Of Hygienic Sciences
-
HARADA MASATOSHI
Division of Pharmacognosy and Phytochemistry, National Institute of Health Sciences
-
Fujimori Kannosuke
Division Of Pharmacology National Institute Of Hygienic Sciences
-
TAKANAKA Akira
Division of Pharmacology, National Institute of Hygienic Sciences
-
Ozaki Yukihiro
Division of Pharmacognosy and Phytochemistry , National Institute of Health Sciences
-
Watano Tomokazu
Division of Pharmacology, National Institute of Hygienic Sciences
-
Ohara-Imaizumi Mica
Division of Pharmacology, National Institute of Hygienic Sciences
関連論文
- EFFECT OF CARBAMATES ON RAT HEPATOCYTES IN THE PRIMARY CULTURE
- EFFECT OF CARBAMATES ON RAT FRESHLY ISOLATED HEPATOCYTES
- THE EFFECT OF DIQUAT ON THE GAP JUNCTIONAL INTERCELLULAR COMMUNICATION BETWEEN RAT HEPATOCYTES IN PRIMARY CULTURE
- EFFECT OF ORGANOPHOSPHORUS COMPOUNDS AND THEIR METABOLITES ON RAT FRESHLY ISOLATED HEPATOCYTES
- EFFECTS OF ORGANIC PHOSPHOROUS COMPOUNDS AND THEIR METABOLITES ON RAT HEPATOCYTES IN THE PRIMARY CULTURE
- Study of the Accelerating Effect of Shikonin and Alkannin on the Proliferation of Granulation Tissue in Rats
- Modulation of Voltage-Gated Ca^ Current by 4-Hydroxynonenal in Dentate Granule Cells(Highlighted paper setected by Editor-in-cfiief)(Pharmacology)
- Sexing of postimplantation rat embryos in stored two-dimensional electrophoresis (2-DE) samples by polymerase chain reaction (PCR) of an Sry sequence
- EFFECT OF PHOSPHORIC ACID TRI-ESTERS FLAME RETARDANTS ON THE PRENATAL AND POSTNATAL DEVELOPMENTS OF THE RATS
- STUDY ON THE SPERM MOTILITY, COUNT OR MORPHOLOGICAL CHANGE AND MALE FERTILITY DISTURBANCE INDUCED BY CHEMICALS
- ATP-and Adenosine-Mediated Signaling in the Central Nervous System : Synaptic Purinoceptors : the Stage for ATP to Play Its "Dual-Role"
- A-1 MECHANISM UNDERLING NEUROTOXICITY OF CHLORDECONE : AN ELECTROPHYSIOLOGICAL APPROACH USING PC12 PHEOCHROMOCYTOMA CELLS
- STUDIES ON NITROSAMINE FORMATION BY THE INTERACTION BETWEEN DRUGS AND NITRITE. I. : Measurement of the amount of nitrosamine formed in rat and guinea pig stomachs.
- Application of the PKCYP Test to Predict Caffeine Clearance Mediated by CYP1A2 in a Rat Acute Liver Injury Model
- Simultaneous Assessment of the In Vivo Amount of CYP1A2 and CYP3A2 by the PKCYP-test Using Theophylline in Rats
- Application of the PKCYP-test to Predict the Amount of in Vivo CYP2C11 Using Tolbutamide as a Probe
- Application of the PKCYP-test in Cases of Altered CYP1A2 for Multiple CYP Systems in Rat Models of Disease
- A Simplified Diagnostic Approach for Estimating in vivo Hepatic Drug Clearance ; Its Preliminary Application for the Drug Caffeine, Using CYP1A Probe in a Rat Model
- Antiinflammatory Effect of Trichosanthes kirilowii MAXIM. and Its Effective Parts
- Accelerative Effect of "Nanshikon"and Its Constituents on the Proliferation of Granulation Tissue in Rats
- STUDY OF EXAMINATION METHODS FOR ASSESSING MALE FERTILITY DISTURBANCES 1.QUANTITATIVE MORPHOMETRICAL EVALUATION OF TESTICULAR TOXICITY OF ADRIAMYCIN
- Determination of Arecoline and Arecaidine in Areca by Reversed-Phase, Ion-Pair High-Performance Liquid Chromatography
- LOSS OF VIABILITY AFTER DISULFIRAM TREATMENT WITHOUT PRECEDING DEPLETION OF INTRACELLULAR GSH
- ORIGIN AND CHARACTERISTICS OF FRESHLY ISOLATED RENAL CELLS OBTAINED BY PERFUSION OF COLLAGENASE IN VITRO
- MORPHINE METABOLISM IN ISOLATED HEPATOCYTES AND ITS IMPLICATIONS FOR HEPATOTOXICITY
- COMPARISONS OF THE TOXICOKINETIC PARAMETERS IN RATS DETERMINED FOR LOW AND HIGH DOSE OF γ-CHLORDANE
- FETAL TOXICITY OF ZINC ETHYLPHENYLDITHIOCARBAMATE(ZEPC)(3). MECHANISM OF FORMATION OF METHEMOGLOBIN CAUSED BY ZEPC
- COMPARATIVE STUDY ON METABOLISM OF N-NITROSODIMETHYLAMINE AND N-NITROSOMETHYLBENZYLAMINE IN RAT LIVER
- RENAL MICROSOMAL N-NITROSODIMETHYLAMINE DEMETHYLASE DETERMINED BY A SENSITIVE RADIOMETRIC METHOD
- INFLUENCE OF DOSE ON THE TOXICOKINETICS OF γ-CHLORDANE
- STUDIES ON NITROSAMINE FORMATION BY THE INTERACTION BETWEEN DRUGS AND NITRITE. II. : Hepatotoxicity by the simultaneous administration of several drugs and nitrite.
- Antiinflammatory Effect of Forsythia suspensa VAHL and Its Active Fraction
- Effects of Tamoxifen on L-Glutamate Transporters of Astrocytes
- Developmental toxicity of indium : Embryotoxicity and teratogenicity in experimental animals
- 219 EFFECTS OF 2-MONOCHLORODIBENZOFURAN AND 3-MONOCHLORODIBENZOFURAN ON THE P450-MEDIATED DRUG METABOLIZING ENZYME ACTIVITIES OF PRIMARY CULTURED RAT HEPATOCYTES : COMPARISION WITH THE EFFECTS OF DIOXINS AND METHLCHOLANTHRENE
- 215 MUTAGENIC ACTIVATION OF 2- AND 3-CHLORODIBENZOFURANS BY LIVER MICROSOMES AND THE CORRELATION WITH CYTOCHROME P-450 ISOZYMES
- A04 EFFECTS OF METYRAPONE ON THE CYTOCHROME P450 MEDIATED DRUG METABOLIZING ACTIVITIES IN THE PRIMARY CULTURES OF RAT HEPATOCYTES
- STUDY OF EXAMINATION METHODS FOR ASSESSING MALE FERTILITY DISTURBANCES 2.THE SPERM EVALUATION USING ADRIAMYCIN
- EFFECTS OF DEPLETION OF EMBRYONIC GLUTATHIONE ON SELENIUM TERATOGENICITY IN CULTURED RAT EMBRYOS
- EFFECTS OF VARIOUS INDUCERS ON THE ACTIVITIES OF CYTOCHROME P-450-MIXED FUNCTION OXIDASES AND AFLATOXIN B_1 ACTIVATION IN MICROSOMES OF HAMSTER LIVERS
- ATP Receptors for the Protection of Hippocampal Functions
- IN VITRO NEUROTOXICITY ASSAY SYSTEM BY NEURONAL CLONES (1) : EFFECTS OF CHLORDANE ON ELECTROPHYSIOLOGICAL PROPERTIES OF HYBRID NEURONAL CLONE NG 108-15
- ATP-and Adenosine-Mediated Signaling in the Central Nervous System : Chronic Pain and Microglia : Involvement of the ATP Receptor P2X_4
- Studies on an Establishment of Cells Expressing Human Vasopressin Receptpr and Its Characterlzation.
- A-15 IN VITRO TERATOGENICITY TESTING USING THE RAT EMBRYO CULTURE SYSTEM : (3) EFFECTS OF CYCLOPHOSPHAMIDE ON RAT EMBRYONIC DEVELOPMENT IN VITRO AND IN VIVO
- A-6 EFFECT OF BIS (TRIBUTYLTIN) PHTHALATE ON THE FETAL DEVELOPMENT
- C12 IN VITRO TERATOGENICITY TESTING USING THE RAT EMBRYO CULTURE SYSTEM : (1) EFEECTS OF SODIUM SALICYLATE ON RAT EMBRYONIC DEVELOPMENT IN VITRO AND IN VIVO
- C03 SYNERGIC EFFECT OF MESTRANOL ON VIRILIZING ACTIVITY OF NORETHYNODREL IN FEMALE RAT FETUSES
- EFFECT OF INTRAMUSCULAR INJECTION OF DRUGS ON SERUM CPK ACTIVITY IN RABBITS
- TERATOLOGICAL STUDIES OF ZINC ETHYLPHENYLDITHIOCARBAMATE (ZEPC), A VULCANIZATION ACCELERATOR FOR RUBBERS, IN RATS
- 218 EFFECTS OF 2- AND 3-CHLORODIBENZOFURANS ON THE DEVELOPMENT OF CULTURED POSTIMPLANTATION RAT EMBRYOS
- Two Non-steroidal Anti-inflammatory Drugs, Niflumic Acid and Diclofenac, Inhibit the Human Glutamate Transporter EAAT1 Through Different Mechanisms
- Antiinflammatory Effect of Forsythia suspensa VAHL and Its Active Principle
- EFFECT OF PHENYLPYRAZOLONE DERIVATIVES ON GASTRIC EMPTYING RATE AND DRUG ABSORPTION IN RATS
- Block by Phytoestrogens of Recombinant Human Neuronal Nicotinic Receptors
- Prevention of Stroke by a Japanese Traditional Medicine, Shichimotsu-koka-to, in Stroke-Prone Spontaneously Hypertensive Rats : a Histopathological Study
- Histological Study of the Accelerating Effect of Shikonin and Alkannin on the Proliferation of Granulation Tissue in Rats
- Determination of Arecoline and Arecaidine in Areca by Reversed-Phase, Ion-Pair High-Performance Liquid Chromatography
- Cholagogic Action of the Essential Oil Obtained from Curcuma xanthorrhiza ROXB.
- Dissolution Tests of Coptis Rhizome and Cultured Cells of Coptis japonica
- Quantitative Analysis of Plant Hormones, Auxins, in Biotechnologically Cultured Products of Medicinal Plants
- Quantitative Studies on Terpenes of Japanese and European Valerians
- Antiinflammatory Effect of Glycyrrhizin Topically Applied to the Cotton Pellet by Granuloma Porch Method in Rats
- Sustained Changes in Acetylcholine and Amino Acid Contents of Brain Regions Following Microsphere Embolism in Rats.
- Antiinflammatory effect of mace, aril of Myristica fragrans Houtt., and its active principles.
- Effects of Tamoxifen on L-Glutamate Transporters of Astrocytes
- Inhibition of Ion Channels by Hirsutine in Rat Pheochromocytoma Cells.
- Non-Competitive Antagonism by Hirsuteine of Nicotinic Receptor-Mediated Dopamine Release from Rat Pheochromocytoma Cells.