WRN Functions in a RAD18-Dependent Damage Avoidance Pathway(Molecular and Cell Biology)

概要

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Werner syndrome (WS), caused by mutations in a gene (WRN) that encodes a RecQ DNA helicase, is characterized by premature aging and cancer predisposition. Cells derived from WS patients show sensitivity to several DNA damaging agents. Previous studies revealed that the WRN protein plays roles in DNA repair or damage tolerance, although it was not yet assigned to a specific pathway. Here we examined the relationship between WRN and the post-replication repair protein RAD18 by generating deletion derivatives in chicken DT40 cells. The frequency of spontaneous sister chromatid exchange in WRN^<-l->/RAD18^<-l-> double mutant cells was slightly increased compared to that of either single mutant. However, the sensitivity of WRN^<-l->/RAD18^<-l-> cells to 4-nitroquinoline 1-oxide and methyl methanesulfonate was almost the same as that of RAD18^<-l-> cells. Moreover, the cisplatin sensitivity of RAD18^<-l-> cells was slightly suppressed by disruption of WRN. These data suggest that WRN functions in a pathway involving RAD18 under damage-inducing conditions.
公益社団法人日本薬学会の論文
2007-06-01