Preservative solution for freeze-storage of surgically excised human colon to enable study of smooth muscle function in vitro
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概要
- 論文の詳細を見る
We have compared the reactivity to carbachol and high potassium of circular smooth muscle isolated from segments of human colon which was freeze-stored in different preservative solutions for more than one month following surgical resection. Concentration-dependent contractions in response to carbachol were reduced in terms of both their sensitivity (pEC_<50>) and reactivity (Emax), depending on the preservative solutions used. Similar reduction of reactivity to 100 mM KC1 was also observed. The best responsivcness was shown when the tissue was freeze-stored in SFM101. It is concluded that the freeze-storage of surgically excised human colon in SFM101 or phosphate buffer solution for more than one month provided the best preservation of smooth muscle function for in vitro pharmacological examination.
- 日本平滑筋学会の論文
著者
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OHNO Yasuo
Division of Pharmacology, National Institute of Health Science
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UCHIDA Kohsuke
Department of Urology, Sapporo Medical University School of Medicine
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Kamikawa Yuichiro
Department of Pharmacology, Dokkyo University School of Medicine
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SHIBUKAWA Asako
Department of Pharmacology Dokkyo University School of Medicine
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Uchida Kohsuke
Dep. Of Pharmacology Dokkyo Medical Univ. School Of Medicine
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Uchida Kohsuke
Department Of Pharmacology Dokkyo Medical University
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SASAKI Kinro
Surgery,Departments of Pharmacology,
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SUNAGAWA Masakatu
Surgery,Departments of Pharmacology,
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Ohno Yasuo
Division Of Internal Medicine And Thoracic Oncology National Cancer Center Hospital
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Sasaki Kinro
Surgery Departments Of Pharmacology
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Shibukawa Asako
Departments Of Pharmacology Dokkyo University School Of Medicine
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Kamikawa Yuichiro
Dep. Of Pharmacology Dokkyo Medical Univ. School Of Medicine
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Kamikawa Yuichiro
Department Of Pharmacology Dokkyo Medical University School Of Medicine
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Ohno Yasuo
Division Of Pharmacology National Institute Of Hygienic Sciences
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Sunagawa Masakatu
Surgery Departments Of Pharmacology
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Uchida Kohsuke
Department Of Pharmacology And Toxicology Dokkyo Medical University School Of Medicine
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