Modification of 5 -Hydroxytryptophan-Evoked 5 -Hydroxytryptamine Formation of Guinea Pig Colonic Mucosa by Reactive Oxygen Species
スポンサーリンク
概要
- 論文の詳細を見る
We studied whether reactive oxygen species (ROS) generated by normal colonic mucosa affect 5-hydroxytryptophan (5-HTP)-evoked 5-HT formation (measured as the sum of 5-HT plus 5-hydroxyindole acetic acid (5-HIAA) accumulation) of guinea pig’s isolated colonic mucosa. Catalase (3000 – 6000 U/ml), a hydrogen peroxide (H2O2) scavenger or diphenylene iodonium (DPI, 10 – 100 μM), an NADPH oxidase inhibitor, concentration-dependently caused an increase of the sum of 5-HT plus 5-HIAA accumulation in the presence of 5-HTP (10 μM), but these drugs did not significantly affect the 5-HT-metabolite in the colonic mucosa measured as the ratio of 5-HIAA/5-HT. Exogenously applied H2O2 (10 – 100 μM) concentration-dependently inhibited the sum of 5-HT plus 5-HIAA accumulation. In contrast, neither superoxide dismutase (SOD, 100 – 300 U/ml), superoxide anion scavenger, nor dimetyl sulfoxide (1 – 5%, DMSO), a hydroxyl radical scavenger affected the sum of 5-HT plus 5-HIAA accumulation. Moreover, mucosa ROS generation was estimated using the chemiluminescence technique. SOD (100 – 300 U/ml), catalase (3000 – 6000 U/ml) or DPI (10 – 100 μM), concentration-dependently reduced luminol-enhanced chemiluminescence signal from the colonic mucosa, while allopurinol (10 – 100 μM), a xanthine oxidase inhibitor, did not affect the chemiluminescence signal. These results suggest that ROS is formed through an NADPH oxidase system in the guinea pig colonic mucosa, where it exerts a modulatory effect on mucosal 5-HT formation upon addition of 5-HTP. Thus, ROS formation from normal colonic mucosa could be considered to contribute to the control of 5-HT production in mucosa enterochromaffin cells.
- 社団法人 日本薬理学会の論文
- 2002-01-01
著者
-
Ikeda Masashi
Institute Of International Education And Research Dokkyo Medical University School Of Medicine
-
Ikeda Masashi
Inst. Of International Education And Res. Dokkyo Medical Univ. School Of Medicine Jpn
-
Ikeda M
Institute Of International Education And Research Dokkyo Medical University School Of Medicine
-
Ikeda Masashi
Institu Of International Education And Research Dokkyo Medical University
-
Kamikawa Yuichiro
Department of Pharmacology, Dokkyo University School of Medicine
-
KOJIMA Shu-ichi
Department of Pharmacology, Dokkyo Medical University School of Medicine
-
SHIBUKAWA Asako
Department of Pharmacology Dokkyo University School of Medicine
-
Ikeda M
Graduate School Of Health Sciences University Of Shizuoka
-
Kojima S
Department Of Pharmacology Dokkyo Medical University School Of Medicine
-
Kojima Shu-ichi
Department Of Engineering Science Kyoto University
-
Kamikawa Y
Department Of Pharmacology Dokkyo Medical University School Of Medicine
-
Kamikawa Yuichiro
Department Of Pharmacology Dokkyo Medical University School Of Medicine
-
Kamikawa Yuichiro
Department of Pharmacology, Dokkyo Medical University School of Medicine
関連論文
- OJ-105 Diversity of Molecular Forms of BNP in Heart Failure : Difference of Molecular Form of BNP in Atrial and Ventricular Overload(OJ18,Heart Failure (Laboratory/Biomarkers) (M),Oral Presentation (Japanese),The 73rd Annual Scientific Meeting of The Japa
- PACAPの側脳室内投与がプロラクチン分泌に与える影響--成熟雄ラットおよび泌乳ラットを用いた検討
- Airway Remodeling in a Guinea Pig Model of Chronic Asthma : Its Influence on Airway Responsiveness and Pharmacological Properties of Airway Smooth Muscle, and Its Prevention by Corticosteroids
- Anticoagulant and fibrinolytic effects of functional food materials produced by Bacillus subtilis natto
- A Newly Derived Protein From Bacillus subtilis natto With Both Antithrombotic and Fibrinolytic Effects
- Further Investigation Into the Mechanism of Tachykinin NK_2 Receptor-Triggered Serotonin Release From Guinea-Pig Proximal Colon
- Loperamide Inhibits Tachykinin NK_3-Receptor-Triggered Serotonin Release Without Affecting NK_2-Receptor-Triggered Serotonin Release From Guinea Pig Colonic Mucosa
- Loperamide Inhibits Tachykinin NK3-Receptor-Triggered Serotonin Release Without Affecting NK2-Receptor-Triggered Serotonin Release From Guinea Pig Colonic Mucosa
- Modification of 5 -Hydroxytryptophan-Evoked 5 -Hydroxytryptamine Formation of Guinea Pig Colonic Mucosa by Reactive Oxygen Species
- Investigation Into the 5-Hydroxytryptophan-Evoked Luminal 5-Hydroxytryptamine Release From the Guinea Pig Colon
- Muscularis mucosae : the forgotten sibling
- Disturbance of Circadian Rhythm in Heart Rate, Blood Pressure and Locomotive Activity at the Stroke-Onset in Malignant Stroke-Prone Spontaneously Hypertensive Rats
- Phospholipid Metabolism in Platelets from Stroke-Prone Spontaneously Hypertensive Rats and Wistar Kyoto Rats
- Impairment of Endothelium-Dependent Relaxation by Diesel Exhaust Particles in Rat Thoracic Aorta
- Preservative solution for freeze-storage of surgically excised human colon to enable study of smooth muscle function in vitro
- Effects of Cyclophosphamide on the Kaolin Consumption (Pica Behavior) in Five Strain Adult Male Rats
- Angular Dependence of Sputtering Yield of Au on Bombardment by 15 to 60 keV Ar^+
- Calcitonin Gene : Related Peptide Inhibits Nonadrenergic Noncholinergic Contraction of Guinea Pig Distal Colon
- AUGMENTATION OF THE VAGALLY-MEDIATED RESPONSE TO 5-HYDROXYTRYPTAMINE IN THE ANAPHYLACTICALLY-DESENSITIZED ATRIA OF GUINEA-PIGS "IN VITRO"
- In vitro Comparison of the Intrinsic Activity of Functional Antagonism between β-agonists and Spasmogens in the Guinea-pig Tracheal Muscle
- Assessments of Student Fitness to Practice among Nursing Students