Electrokinetic Evaluation of Individual Exosomes by On-Chip Microcapillary Electrophoresis with Laser Dark-Field Microscopy
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概要
- 論文の詳細を見る
Cell-secreted nanovesicles called exosomes are expected as a promising candidate biomarker of various diseases. Toward the future application of exosomes as a disease biomarker for low-invasive diagnostics, challenges remain in the development of sensitive and precise analysis methods for exosomes. In this study, we performed the electrokinetic evaluation of individual exosomes by the combined use of on-chip microcapillary electrophoresis and laser dark-field microscopy. We extracted exosomes from six types of human cell cultured in a serum-free medium by differential ultracentrifugation and their zeta potential (electrophoretic mobility) were evaluated. We demonstrated that the proposed electrophoresis apparatus is particularly suitable for the tracking analysis of the electrophoretic migration of individual exosomes and enables the accurate evaluation of the zeta potential distribution of exosomes, for the first time. From the experimental results, we found that there is a strong correlation between the average zeta potentials of exosomes and their cells of origin.
- 2013-06-25
著者
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Kato Kei
Department Of Applied Chemistry Faculty Of Engineering Gunma University
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Kobayashi Masashi
Department Of Clinical Pharmacology Toyama Medical & Pharmaceutical University
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Akagi Takanori
Department of Bioengineering, School of Engineering, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-8656, Japan
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Ichiki Takanori
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Kato Kei
Department of Bioengineering, School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Hanamura Nami
Department of Bioengineering, School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Kosaka Nobuyoshi
Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Chuo, Tokyo 104-0045, Japan
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Ochiya Takahiro
Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Chuo, Tokyo 104-0045, Japan
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Akagi Takanori
Department of Bioengineering, School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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