Application of On-Chip Electrophoresis of Cell to Evaluation of Cell Cycle Stages of HL-60 Cells
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概要
- 論文の詳細を見る
In this article, we report a new method of cell cycle stage typing using a microcapillary electrophoresis (μCE) chip. Cell electrophoretic mobility (EPM) is considered to be useful information for noninvasive cell state analysis because EPM reflects the surface properties of cells. Human leukemic cells (HL-60) were synchronized at certain phases of the cell cycle using various synchronizing drugs, and their EPMs were measured using a μCE chip. It was found that the EPM distribution of the synchronized cells is much narrower than that of the nonsynchronized cells, and that the peak value of the EPM distribution differs between all cell cycle stages. These results suggest that on-chip EPM measurement is a promising method of cell cycle stage typing.
- Japan Society of Applied Physicsの論文
- 2006-10-25
著者
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Ichiki Takanori
Department of Bioengineering, School of Engineering, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-8656, Japan
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Akagi Takanori
Department of Bioengineering, School of Engineering, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-8656, Japan
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Suzuki Masae
Institute of Engineering Innovation, School of Engineering, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-8656, Japan
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Ichiki Takanori
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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