Microintaglio Printing of Biomolecules and Its Application to In situ Production of Messenger Ribonucleic Acid Display Microarray
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概要
- 論文の詳細を見る
A new molecular printing technology for a simple and robust patterning of biomolecules was developed and applied to producing a genotype-linked protein microarray. A microarray of messenger ribonucleic acids (mRNAs) encoding the green fluorescent protein was patterned by microintaglio printing using a micromold comprising an array of uniformly arranged 5-μm-diameter holes at a density of 10,000 per mm2. Furthermore, one-step conversion from an mRNA microarray into an mRNA-protein fusion microarray was performed by simultaneous cell-free protein synthesis and fusion reaction using a puromycin-labeled oligonucleotide linker. The set of developed technologies provides a powerful means of in vitro protein evolution.
- 2011-04-25
著者
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OSAWA Terutsune
Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu Univers
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ICHIKI Takanori
Department of Electrical & Electronics Engineering, Toyo University
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Biyani Manish
Create-jst
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Biyani Manish
Department Of Functional Materials Science Saitama University
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Osawa Terutsune
Department Of Functional Polymer Science Faculty Of Textile Science And Technology Shinshu Universit
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Nemoto Naoto
Japan Science and Technology Agency, CREST, Chiyoda, Tokyo 102-0075, Japan
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Biyani Manish
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Ichiki Takanori
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Osawa Terutsune
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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