On-Chip Evaluation of Damage on Cell Surfaces Induced by Cell Dissociation Agents
スポンサーリンク
概要
- 論文の詳細を見る
The application of on-chip cell electrophoresis to the diagnosis of cell damage after treatment with a cell dissociation agent has been studied for the first time using both floating and adherent cells. First, a preliminary experiment was carried out using HL-60 (human leukemic) cells in order to investigate how cell dissociation agents affect the cell surface. After the treatment of HL-60 cells with three different cell dissociation agents for 20 min, the effects of cell dissociation agents on the cell were examined by both biological and electrical approaches. The result of an on-chip electrophoresis experiment showed the possibility of detecting a slight change in HL-60 cell surfaces, while no significant changes were observed by biological methodologies. The largest effect was particularly observed for trypsin treatment. Subsequently, the effects of trypsin treatment time were investigated using HeLa cells as an example of adherent cells. As a result, both biological and electrical measurements showed an increasing variation of the cells with trypsin treatment time. Thus, it is considered that the possible application of an on-chip cell electrophoretic mobility measurement system for the noninvasive evaluation of cell state that does not include cell labeling or mechanical damage after cell dissociation agent treatment. Such a system is expected to be valuable for the quality control of sample cells that will be required in future cell-based biotechnology applications.
- 2007-09-30
著者
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Ichiki Takanori
Department of Bioengineering, School of Engineering, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-8656, Japan
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Akagi Takanori
Department of Bioengineering, School of Engineering, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-8656, Japan
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Suzuki Masae
Institute of Engineering Innovation, School of Engineering, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-8656, Japan
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Ichiki Takanori
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Sato Takahiro
Biomaster Inc., 3-35 Minaminakadori, Naka-ku, Yokohama 231-0006, Japan
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