Laser-induced Stress Wave-assisted Gene Transfection with Cationic Liposome
スポンサーリンク
概要
- 論文の詳細を見る
We showed that efficiency of gene transfer using laser-induced stress waves (LISWs) can be drastically enhanced by modifying plasmid DNA with cationic liposome. Cultured NIH 3T3 cells were covered with a suspension including plasmid DNA that had been modified with Lipofectamine, and a laser target was placed over the suspension. The cells were exposed to stress waves generated by irradiation of the laser target with 6-ns laser pulses. The cells showed a significantly higher transfection efficiency compared with that of cells treated with LISWs alone or Lipofectamine alone.
- 2007-12-25
著者
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Obara Minoru
Department Of Electrical Engineering Faculty Of Engineering Keio University
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Ashida Hiroshi
Division Of Biomedical Information Sciences National Defense Medical College Research Institute
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Sato Shunichi
Division Of Biomedical Information Sciences National Defense Medical College Research Institute
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Takishima Kunio
Department Of Biochemistry I National Defense Medical College
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Terakawa Mitsuhiro
Department Of Electronics And Electrical Engineering Keio University
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Okano Hideyuki
Department Of Molecular Neurobiology Institute Of Basic Medical Sciences And Center For Tara(tsukuba
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Satoh Yasushi
Department Of Anesthesiology National Defense Medical College
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Terakawa Mitsuhiro
Department of Electronics and Electrical Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan
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Otsuka Risa
Department of Electronics and Electrical Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan
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Takishima Kunio
Department of Biochemistry, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan
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Satoh Yasushi
Department of Biochemistry, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan
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Okano Hideyuki
Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan
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