In Vivo Targeted Gene Transfer by Direct Irradiation with Nanosecond Pulsed Laser
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概要
- 論文の詳細を見る
We demonstrated in vivo targeted gene transfer to rat skin by direct irradiation with nanosecond laser pulses without major side effects. Expressions of enhanced green fluorescent protein (EGFP) were observed only in the area irradiated with laser pulses; in the skin, epidermal cells were selectively transfected. Unlike other physical methods, this method enables noncontact gene transfer. Moreover, the laser intensity required in this method is as low as 20 MW/cm2, and thus fiber-based beam delivery is possible.
- Japan Society of Applied Physicsの論文
- 2004-10-01
著者
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Obara Minoru
Department Of Electrical Engineering Faculty Of Engineering Keio University
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Ashida Hiroshi
Division Of Biomedical Information Sciences National Defense Medical College Research Institute
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Sato Shunichi
Division Of Biomedical Information Sciences National Defense Medical College Research Institute
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Ogura Makoto
Department Of Electronics And Electrical Engineering Keio University
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Ogura Makoto
Department of Electronics and Electrical Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan
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Ogura Makoto
Department of Chemistry, Faculty of Science, Kumamoto University
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