A method for gene transfer, single isolation and in vitro assay for neural stem cells
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概要
- 論文の詳細を見る
The neurosphere method is widely used to examine self-renewal activity and the multipotency of neural stem cells (NSCs). We developed an efficient method for introducing a transgene into neurospheres using a retroviral vector and isolating a transgenic subpopulation of NSCs labeled with green fluorescent protein (GFP) using fluorescence-activated cell sorting, enabling these cells to be used in subsequent biological assays. This method is expected to be useful for studying the function of genes in NSCs.
- 日本炎症・再生医学会の論文
- 2005-01-25
著者
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OKANO Hideyuki
Department of Physiology, Keio University School of Medicine
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SHIMAZAKI Takuya
Department of Physiology, Keio University School of Medicine
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Kitamura Toshio
Division Of Cellular Therapy Institute Of Medical Science University Of Tokyo
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Shimazaki Takuya
慶応義塾大学 医学部生理学教室
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Shimazaki Takuya
Department Of Physiology Keio University School Of Medicine
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SHIBUYA Akira
Department of Physical Science, Graduate School of Engineering Science, Osaka University
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Kitamura Toshio
Division Of Cellular Therapy Advanced Clinical Research Center Institute Of Medical Science Universi
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Shibuya Akira
Department Of Immunology Institute Of Basic Medical Sciences And Center For Tara Graduate School Of
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Shibuya Akira
Department Of Immunology Institute Of Basic Medical Sciences Graduate School Of Comprehensive Human
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Sawamoto Kazunobu
Department Of Physiology Keio University School Of Medicine
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Sawamoto Kazunobu
Department Of Molecular Neurobiology Institute Of Basic Medical Sciences And Center For Tsukuba Adva
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SAKAGUCHI Masanori
Department of Physiology, Keio University School of Medicine
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Okano Hideyuki
Department Of Neuroanatomy Biomedical Research Center Osaka University Medical School
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Sakaguchi Masanori
Department Of Cardiovascular Surgery Osaka City University Graduate School Of Medicine
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Sakaguchi Masanori
Department Of Physiology Keio University School Of Medicine
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Okano Hideyuki
Department Of Molecular Neurobiology Institute Of Basic Medical Sciences And Center For Tara(tsukuba
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SAWAMOTO Kazunobu
Department of Developmental and Regenerative Biology, Institute of Molecular Medicine, Nagoya City University Graduate School of Medical Sciences
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