Development of an Enzyme-Linked Immunosorbent Assay for the Determination of α<SUB>1</SUB>-Antichymotrypsin in Serum and Cerebrospinal Fluid

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We have developed a sensitive enzyme-linked immunosorbent assay for the determination of arantichymotrypsin (ACT) and measured it in serum and cerebrospinal fluid (CSF) collected from some patients without any probed neurological diseases. Using 5 pl of CSF or 100 times diluted serum, as less as 0.4 μg/ml of ACT could be detected by this method. The coeffi-cients of variation for between-run measurement by this method were 3.8% (n=10, X =6.3μg/ml) for ACT in CSF and 3.7% (n=10, X=451μg/ml) for ACT in serum. The inter-ference by bilirubin, hemoglobin turbidity, albu-min, immunoglobulin G, haptoglobin, α1-acid gly-coprotein and prealbumin was negligible. The normal reference values of ACT (mean±SD) were determined as being 447±85 μg/ml (n=86) for serum and 2.23±0.69μg/ ml (n=11) for CSF.
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