The improvement of a multiwave high performance liquid chromatogra phy detection system for the purpose of diagnosis

概要

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High performance liquid chromatography(HPLC)using a multiwave detector possesses the advantage of being able to measure both the absorption spectra and the retention time. But conventional multiwave detector with diode arrays has lower sensitivity than commercial single wave UV detector with photomultiplier tubes and shows a marked decrease of sensitivity in the UV region below200n m. With our multiwave HPLC detection system, mechanical scanning with a rotary slit is conducted with a photomultiplier that has full sensitivity in the wavelength renge of160-650nm. And absorption due to oxygen below the200nm wavelength region has been inhibited by filling the optical system with nitrogen . This has resulted in an improvement in this system's detection sensitivity enabling it to measure in the UV region below200nm. Using this system, we tried out multiwave HPLC analysis for a number of clinical chemically important saccarides and amino acids without using some coloring reagents. As a result of this it was found possible to detect absorption peaks of each component below a wavelength of200nm, and the possibility of conducting qualitative analysis from the absorption spectra of the components was also demonstrated.
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