Analysis of urine of chronic alcoholic patients by high performance liquid chromatography with multi chammel detector and ion exchange column

概要

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We compared the column chromatograph patterns of alcoholic patients with those of healthy volunteers. Human Urine obtained from healthy male volunteers and alcoholic male patients was analyzed by means of high performance liquid chromatography using ion exchange column(3013-N, Hitachi). The solutions used in the column were ion exchage water(A solution)and 0.25M ammonium perchlorate PH5.8-acetonitrile(4: 1)(B solution). The elutin diagram was from 0% to 20% B solution during 0-40min and from 20% to 100% B solution during 40-60min and 100% B solution during 60-70min and 100% Asolution during 70-100min. Two kinds of detector system were used. By using fixed wavelength we monitored the absorption at 254nm and by using multi-channel detector we adjusted the wavelength range from 200nm to 400nm. With the fixed wavelength the peaks which were not observed in the healthy volunteers appeared in the alcoholic patients and the peaks which were observed in the healthy volunteers disppeared in the alcoholic patients. This result was supported by the findings obtained from the multi channel detector in which the peaks from alcoholic patients disappeared or appeared in other wavelengths.