Determination of Procainamide and N-Acetylprocainamide in Breast Milk and Serum Usiug High-Performance Liquid Chromatography

概要

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A simple, accurate and micro-scale method for the simultaneous determination of procainamide (PA) and N-acetylprocainamide (NAPA) in breast milk and serum using high-performance liquid chromatography is described. Only 50μl of specimen is required. PA and NAPA in the specimen are purified by solid-phase extraction method using CLIN-ELUT and then liquid-liquid back extraction method using dilute phosphoric acid. An aliquot of the acidic aqueous solution is injected directly into the chromatograph. within-run and day-to-day variation for PA at 4μg/ml was below 2.0% and that for NAPA at 12μg/ml was below 1.5%. This method and fluorescence polarization immunoassay have shown a good correlation.<BR>The concentrations of both PA and NAPA in the breast milk were 4.3-6.4 times higher than the maternal serum values. The trace amount of both PA and NAPA was found in serum of an infant fed with milk from mother under PA therapy.
Japan Society of Clinical Chemistryの論文