高速液体クロマトグラフ法による Methotrexate 血中濃度測定法の検討 : 螢光偏光免疫測定法との比較及び骨肉腫患者血液への応用

概要

論文の詳細を見る
We determined the methotrexate (MTX) concentration in sera of 15 patints with osteosarcoma using high performance liquid chromatography (HPLC). The patients were administered high-dose MTX in conjunction with leucovorin rescue therapy. The values obtained by HPLC were compared with those obtained by fluorescence polarization immunoassay (EPIA) (n=47), and a high correlation was observed (1.54×10^<-7>mol/l〜1.70×10^<-3>mol/l). The MTX concentrations determined by FPIA and HPLC at 6, 24, 48 and 72 hours after initiation of the 6-hour intravenous infusion were analyzed by paired t-tests. No Significant difference was observed in the values at 6 and 24 hours between the two methods. However, the values at 48 (p<0.05) and 72 hours (p<0.0001) obtained by FPIA were significantly higher than those obtained by HPLC. The difference may be due to the presence of 7-OH-MTX, a metabolite of MTX, in these sera because the antibody crossreacts with this metabolite in this immunoassay. Caution should be taken in this FPIA interference.
日本医療薬学会の論文
1994-10-20