高速液体クロマトグラフィーによる新生児血漿中パニペネム濃度の測定

概要

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A simple and fast determination of plasma panipenem (PAPM) in extremely low birth weight infants was established using reversed phase high-performance liquid chromatography. Solutions of 35% methanol (pH 5.8), including 5 mM NaH_2PO_4 and 5 mM sodium dodecylsulfate, were used for the mobile phase. The flow rate was 0.8 mL/min. UV detection was carried out at 300 nm. The pretreatment method was as follows : 200 mM 3-[morpholino] propansulfonic acid solution was added to the plasma as a stabilizer. It was then deproteinized with methanol. The calibration curve was linear in a range from 6.25 μg/mL to 100μg/mL. The recovery rate from known concentration samples of PAPM was 98.4%. The within-run and day-to-day variations were below 2.5% and 2.7%, respectively. The PAPM concentrations in the plasma were determined in five infants. In immature patients. an extension of the half-life was also observed.
日本医療薬学会の論文
2000-10-10