Cytochalasin D Inhibits Smooth Muscle Contraction by Directly Inhibiting Contractile Apparatus.
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概要
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We investigated the mode of relaxant effects of cytochalasin D, a capping agent of actin filaments, on contractile responses in the rat aorta and chicken gizzard smooth muscles.Cytochalasin D inhibited the contraction induced by high K<SUP>+</SUP> or noradrenaline (10nM-1μM) without changing cytosolic Ca<SUP>2+</SUP> level ([Ca<SUP>2+</SUP>] i) in the rat aorta. In the absence of external Ca<SUP>2+</SUP>, 12-deoxyphorbol 13-isobutylate (DPB) (1μM) induced sustained contraction without increasing in [Ca<SUP>2+</SUP>] i and cytochalasin D also inhibited this contraction. In the permeabilized chicken gizzard smooth muscle, cytochalasin D inhibited the Ca<SUP>2+</SUP> (1-10μM)-induced contraction. Cytochalasin D also inhibited the Ca<SUP>2+</SUP>-independent contraction in the muscle which had been thiophosphorylated by ATP<SUB>γ</SUB>S. Cytochalasin D decreased the velocity of superprecipitation in the chicken gizzard native actomyosin (myosin B) affecting neither the level of MLC phosphorylation nor Mg<SUP>2+</SUP>-ATPase activity. These results suggest that cytochalasin D inhibits smooth muscle contractions without any effect on the Ca<SUP>2+</SUP>-dependent MLC phosphorylation or subsequent activation of myosin ATPase activity. Based on these evidences, it is concluded that cytochalasin D may inhibit smooth muscle contraction possiblly through uncoupling of the force generation from the activated actomyosin Mg<SUP>2+</SUP>-ATPase.
- 日本平滑筋学会の論文
著者
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Hori Masatoshi
Department Of Radiology Graduate School Of Medicine Osaka University
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Saito Shin-ya
Department Of Pharmaceutical Molecular Bio1ogy Facu1ry Of Pharmaceutical Sciences Tohoku Universiry
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KARAKI Hideaki
Life Sciences, The University of Tokyo
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OZAKI Hiroshi
Life Sciences, The University of Tokyo
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SAITO Shin-ya
Department of Veterinary Pharmacology, Graduate School of Agriculture
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