Isolation and Characterization of Bovine Intestinal Subepithelial Myofibroblasts
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概要
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Intestinal subepithelial myofibroblasts (ISMFs) are mesenchymal cells that exist under the epithelium of intestines. Primarily isolated ISMFs from rodents have been applied to experiments. However, due to the size of their intestines, the available cell number is limited. Thus, we attempted to isolate ISMFs from bovine colon as an alternative material. After detachment of smooth muscle and epithelial layers, colonic mucosa was explanted. After 2-week incubation, α-SMA (+) / vimentin (+) / desmin (−) ISMFs were harvested and applied for experiments. First we examined the effect of cell passage on morphology and proliferation activity of bovine ISMFs. Although, 3rd and 7th passage bovine ISMFs did not exhibit any changes, 11th passage ISMFs showed rounded enlarged shape and lost proliferation potential. On the contrary, rat ISMFs displayed the above senescent changes at earlier passage (passage 4). In intracellular Ca2+ concentration measurement, bioactive substances (0.3 – 1 µM ATP, 0.1 – 1 µM serotonin, 10 – 100 nM endothelin-1, and 1 – 10 nM bradykinin) dose-dependently induced an increase in intracellular Ca2+ concentration in bovine ISMFs (passage 3 and 7). However, at passage 11, impairment in intracellular Ca2+ responses was observed. Thus, bovine ISMFs might be novel useful tools with long life span and good cellular responses to investigate physiological/pathophysiological roles of ISMFs.
- 社団法人 日本薬理学会の論文
- 2010-01-20
著者
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Hori Masatoshi
Department Of Radiology Graduate School Of Medicine Osaka University
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Hori M
Leaf Tobacco Research Center Japan Tobacco Inc.:(present Address)laboratory Of Insect Science And Bi
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Ozaki H
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences University Of
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Ozaki Hiroshi
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences The Universit
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Ina Kenji
Nagoya University Medical School
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Hori M
Division Of Veterinary Pharmacology Nippon Veterinary And Animal Science University
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Iwanaga Koichi
Department Of Ergonomics Faculty Of Engineering Chiba University
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Murata Takahisa
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences The Universit
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Hori Masatoshi
Division Of Veterinary Pharmacology Nippon Veterinary And Animal Science University
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Ozaki Hiroshi
Department Of Electronic Engineering Faculty Of Engineering Osaka University
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Iwanaga Koichi
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences The Universit
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Ozaki Hiroshi
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences The Universit
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