Differential effects of anti-inflammatory agents on lysosomal cysteine proteinases cathepsins B and H from rat spleen.
スポンサーリンク
概要
- 論文の詳細を見る
The reactivity and specificity of commonly used anti-inflammatory agents with lysosomal cysteine proteinases cathepsins B and H purified from rat spleen have been investigated. Of the different agents tested, flufenamic acid and indomethacin were known to be potent inhibitors of cathepsin B. A half-maximal inhibition of the activity of cathepsin B was achieved at drug concentrations of 7.6×10<SUP>-5</SUP> M of flufenamic acid and 4.0×10<SUP>-4</SUP> M of indomethacin. The inhibition by these two agents was of a non-competitive type with benzyloxy-carbonyl-phenylalanyl-arginine-4-methyl-7-coumarylamide (Z-Phe-Arg-MCA) as a substrate. The maximal inhibitory potencies of these agents for the cathepsin B activity were observed at pH 7.0. At pH values between 4.5 and 6.5, the inhibitory potencies were less than at pH 7.0. No preincubation time was needed for the reaction between these agents and cathepsin B. In contrast, cathepsin H was not affected by these two drugs even at the drug concentration of 10<SUP>-3</SUP> M at pH values between 4.5 and 8.0. Other anti-inflammatory agents including aspirin, sodium salicylate, phenylbutazone and prednisolone were found to be poorly or scarcely inhibitory for both cathepsins B and H.
- 公益社団法人 日本薬理学会の論文
著者
-
Yamamoto Kenji
Department Of Cardiovascular Surgery Kanagawa Cardiovascular And Respiratory Center
-
Kato Yuzo
Department of Pharmacology, Nagasaki University School of Dentistry
-
Kato Yuzo
Department of Crystalline Materials Science, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan
-
YAMAMOTO Kenji
Department of Pharmacology, Nagasaki University School of Dentistry
-
KAMATA Osamu
Department of Pharmacology, Faculty of Dentistry, Kyushu University
関連論文
- Novel Surface Processing with Sulfonic Acid for Quantum Dot and Its Characteristics
- On the Cyto-Toxicity Caused by Quantum Dots
- Altered Expression of γ-Glutamylcysteine Synthetase, Metallothionein and Topoisomerase I or II during Acquisition of Drug Resistance to Cisplatin in Human Ovarian Cancer Cells
- No evidence for significant association between GABA receptor genes in chromosome 15q11-q13 and autism in a Japanese population
- Tachykinin 1 (TAC1) gene SNPs and haplotypes with autism : A case-control study
- Serotonin transporter gene promoter polymorphism and autism : A family-based genetic association study in Japanese population
- No association of FOXP2 and PTPRZ1 on 7q31 with autism from the Japanese population
- Cyclic AMP Phosphodiesterase 4 Isoenzyme Inhibitory Activity of (R)- and (S)-Isomer of 7-Methyl- or 8-Alkyl-4, 5, 7, 8-tetrahydroimidazo [2,1-i] -purin-5-one (Highlihted paper slected by Editor-in-chief)(Medicinal Chemistry)
- Synthesis and Cyclic AMP Phosphodiesterase 4 Isoenzyme Inhibitory Activity of Heterocycle Condensed Purines
- Phosphodiesterase 4 Isoenzyme Inhibitory Activity of 3-Phenylxanthines and 4-Phenyl[i]condensed-purines
- Condensed-Purines Syntheses of Tetrahydro-1,4-diazepino[1,2,3-gh]purin-2-one and Hexahydro-1,4-diazocino[1,2,3-gh]purin-2-one
- Structural Analysis of Disaccharides Synthesized by β-D-Glucosidase of Bifidobacterium breve clb and Their Assimilation by Bifidobacteria
- Transglycosylation Activity of β-N-Acetylhexosaminidase from Penixillium pxalicum and Its Application to Synthesis of a Drug Carrier
- Analysis of Low Temperature Inducible Mechanism of γ-Glutamyltranspeptidase of Escherichia coli K-12
- Cloning and Nucleotide Sequence of the β-D-Glucosidase Gene from Bifidobacterium breve clb, and Expression of β-D-Glucosidase Activity in Escherichia coli
- Mapping, Cloning, and DNA Sequencing of pepB Which Encodes Peptidase B of Escherichia coli K-12
- Screening for Bacterial Strains Producing Lactate Oxidase
- Transcriptional Regulation of Tyrosine Phenol-Lyase Gene of Erwinia herbicola AJ2985
- Subunit Association of γ-Glutamyltranspeptidase of Escherichia coli K-12^1
- Formation of Lyso-glycosphingolipids by Streptomyces sp.
- Effect of Site-Directed Mutations on Processing and Activity of γ-Glutamyltranspeptidase of Escherichia coli K-12
- OJ-255 A Possible Role of Periodontitis in Acute Myocardial Infarction(Acute myocardial infarction, clinical (pathophysiology)-2 (IHD) OJ43,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- Characterization of New Fluorogenic Substrates for the Rapid and Sensitive Assay of Cathepsin E and Cathepsin D^1
- Deglycosylated Glucoamylase from Rhizopus niveus is Precipitated by Flavobacterium sp. Endo-β-N-acetylglucosaminidase(Microbiology & Fermentation Industry)
- Angiostatin : An Endogenous Inhibitor of Angiogenesis and Tumor Growth
- Thallium-201 brain SPECT to diagnose aggressiveness of meningiomas
- Differential diagnosis in patients with ring-like thallium-201 uptake in brain SPECT
- Biofeedback Training for Bronchial Asthma (Part I)
- Expression of the β-D-Glucosidase I Gene in Bifidobacterium breve 203 during Acclimation to Cellobiose
- Unique Production of a Disaccharide, Galβ1→3GalNAc, by Endo-α-N-Acetylgalactosaminidase of Alcaligenes sp.
- Preservation of Raw Fish and Meat(Food & Nutrition)
- Peptide Production from Protein without Salt(Microbiology & Fermentation Industry)
- Induction and Efficient Purification of Endo-α-N-acetylgalactosaminidase from Alcaligenes sp.(Microbiology & Fermentation Industry)
- Isolation and Characterization of a Blood Group A Substancedegrading α-N-Acetylgalactosaminidase from an Acremonium sp.
- A Novel End-β-N-acetylglucosaminidase Acting on Complex Oligosaccharides of Glycoproteins in a Fungus (Microbiological Fermentation Industry)
- Effect of Yeast Extract on Endo-β-N-acetylglucosaminidase Production by a Flavobacterium sp.(Microbiology & Fermentation Industry)
- Purification, Characterization, and Sequencing of Two Cysteine Proteinase Inhibitors, Sca and Scb, from Sunflower (Helianthus annuus) Seeds
- Characterization of Phagosomal Subpopulations along Endocytic Routes in Osteoclasts and Macrophages
- An Effect of Appreciating Music on Emotion : Follow Up of the Profile of Mood States Score Before and After Appreciating Music
- The treatment of neuroleptic malignant syndrome using dantrolene sodium
- Biofeedback Training for Bronchial Asthma (Part II)
- On the Trend of Biofeedback Studies as seen in CIM
- An Involvement of Granulocyte Medullasin in Phenytoin-Induced Gingival Overgrowth in Rats
- Effects of localized lesions in the lateral suprasylvian cortex on convergence eye movement in cats
- The Regulation of Bone Resorption in Tooth Formation and Eruption Processes in Mouse Alveolar Crest Devoid of Cathepsin K
- Gingipains Inactivate a Cell Surface Ligand on Porphyromonas gingivalis That Induces TLR2- and TLR4-Independent Signaling
- Rapid Degradation of Cathepsin E in Pre-Golgi Compartments after Tunicamycin Treatment
- Simultaneous Multicolor Detection System of the Single-Molecular Microbial Antigen with Total Internal Reflection Fluorescence Microscopy
- Deglycosylation of Hen Ovalbumin in Native Form by Endo-β-N-Acetylglucosaminidase(Food & Nutrition)
- Electronic and Structural Requirements for Metabolic Activation of Butylated Hydroxytoluene Analogs to Their Quinone Methides, Intermediates Responsible for Lung Toxicity in Mice
- Purification and Characterization of a Protease-Resistant Cellulase from Aspergillus niger
- Primary Structure of an O-Linked Sugar Chain Derived from Glucose Oxidase of Aspergillus niger(Biological Chemistry)
- The Release of Oligosaccharides from Glycoproteins by Endo-β-N-Acetylglucosaminidase of Flavobacterium sp.
- Elucidation of the Role of Sugar Chains in Glycosylated-enzymes Using Endo-β-N-acetylglu-cosaminidase from Flavo-bacterium sp.
- Cathepsin E-Deficient Mice Show Increased Susceptibility to Bacterial Infection Associated with the Decreased Expression of Multiple Cell Surface Toll-Like Receptors
- Association of Cathepsin E Deficiency with Development of Atopic Dermatitis
- Porphyromonas gingivalis Proteinases as Virulence Determinants in Progressin of Periodonatal Diseases
- Biochemical and Functional Properties of Lysine-Specific Cysteine Proteinase(Lys-Gingipain)as a Virulence Factor of Porphyromonas gingivalis in Periodontal Disease^1
- Cloning and Sequencing of the Gene Encolding a Novel Lysine-Specific Cysteine Proteinase (Lys-Gingipain)in Porphyromonas gingivalis: Structural Relationship with the Arginine-Specific Cysteine Proteinase(Arg-Gingipain)
- Biochemical Properties of the Monomeric Mutant of Human Cathepsin E Expressed in Chinese Hamster Ovary Cells: Comparison with Dimeric Forms of the Natural and Recombinant Cathepsin E
- Structure-Activity Relationships of Alkylxanthine Inhibitors of Phosphodiesterase IV Isoenzyme
- Novel Specificities of Mucor hiemalis Endo-β-N-acetylglucosaminidase Acting Complex Asparagine-Linked Oligosaccharides
- Up-regulation, Enhanced Maturation, and Secretion of Cathepsin E in Mouse Macrophages Treated with Interferon-γ or Lipopolysaccharide
- The Role of the Cathepsin E Propeptide in Correct Folding, Maturation and Sorting to the Endosome
- Mutational Analysis of Residues in Two Consensus Motifs in the Active Sites of Cathepsin E
- Design and Synthesis of Sensitive Fluorogenic Substrates Specific for Lys-Gingipain^1
- Multiple Cavernous Hemangiomas of the Skull Associated With Hepatic Lesions : Case Report
- The Effect of Magnesium Ion on Yeast UDP-N-acetylglucosamine Pyrophosphorylase and Its Activation by Dithiothreitol
- A Novel Type of Two-Component Regulatory System Affecting Gingipains in Porphyromonas gingivalis
- Selective Proteolysis of Apolipoprotein B-100 by Arg-Gingipain Mediates Atherosclerosis Progression Accelerated by Bacterial Exposure
- Fragile X carrier screening and FMR1 allele distribution in the Japanese population
- Activation of Endo-β-N-Acetylglucosaminidase from Arthrobacter protophormiae by Various Sugars
- PROJECTIVE TRANSFORMATION TO ALIGN MULTI-CAMERA IMAGES FOR EFFICIENT FTV COMPRESSION(International Workshop on Advanced Image Technology 2005)
- Quantum Dots Targeted to the Assigned Organelle in Living Cells
- Novel non-apoptotic morphological changes in neurons of the mouse hippocampus following transient hypoxic-ischemia
- Application of the relaxation technique in general hospital psychiatry
- Effects of Size of Carbohydrate Chain on Protease Digestion of Aspergillus niger Endo-β-I,4-glucanase
- Delivery outcomes of gasless laparoscopic ovarian cystectomy during pregnancy(Session14 Others3)
- Purification and Properties of Acinetobacter sp. Endo-β-N-Acetylglucosaminidase Acting on Complex Olgosaccharides of Glycoproteins
- Purification and Characterization of a Novel Fungal Endo-β-N-acetylglucosaminidase Acting on Complex Oligosaccharides of Glycoproteins(Microbiology & Fermentation Industry)
- Purification and Characterization of Endo-α-N-acetylgalactosaminidase from Alcaligenes sp.(Microbilolgy & Fermentation Industry)
- Isolation of Endo-α-N-acetylgalactos-aminidase from an Aerobic Bacterium(Microbiology & Fermentation Industry)
- Surgical results of low-dose systemic heparinization 1mg/kg, 100IU/kg) in thoracic aortic operation combined with a heparin-coated cardiopulmonary bypass circuit : Is low-dose heparinization safe during deep hypothermic circulatory arrest?
- Cooperative Action of Arg- and Lys-gingipains in Disruption of Fibronectin-Integrin Interactions in Human Gingival Fibroblasts
- Low Temperature Vapor Phase Epitaxy of Undoped ZnSe Films on (100) GaAs Using Metallic Zn and Se
- Suppression of Gingival Inflammation Induced by Porphyromonas gingivalis in Rats by Leupeptic
- New Ligands with a Wide Bite Angle. Efficient Catalytic Activity in the Rh(I)-Catalyzed Hydroformylation of Olefins
- Purification and Characterization of β-D-Glucosidase (β-D-Fucosidase) from Bifidobacterium breve clb Acclimated to Cellobiose
- Quantitative Analysis of the Growth of Bacillus circulans on a Slant Agar Medium
- Photodegradative Surfactants : Photolysis of p-Dodecylbenzyltrimethylammonium Bromide in Aqueous Solution
- Monitoring the Transcription and Transport Process of RNA Using Optical Imaging Techniques: A Brief Review
- LECT2 intron/exson structure : potential strategies for bio-imaging
- Collective Fluorescence Oscillation in a Water Dispersion of Colloidal Quantum Dots
- Approaches to the treatment of somatoform disorders in different settings : Liaison psychiatry
- Photodegradative Surfactants : p-Dodecylbenzyltrimethylammonium Bromide as a Photodegradative Emulsifier for Microemulsion Polymerization
- Condensation Reactions of Amino Acids under Hydrothermal Conditions with Adiabatic Expansion Cooling
- High-Density Diagnostics for Laser-Imploded Plasmas
- A Quantum Scattering Approach to the Level Shift of Spectral Lines in Plasmas
- On the Complex Level Shift of Spectral Lines in Plasmas
- Lanthanide Ion as a Catalyst for Internucleotide Bond Formation