Effect of Bacteriophage in Enterotoxigenic Escherichia coli (ETEC) Infected Pigs
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概要
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We evaluated effect of enterotoxigenic Escherichia coli (ETEC) specific lytic phage CJ12 in ETEC infected pigs. Phage was mixed with feed at a ratio of 1:1,000 (0.1%). One week after initially providing phage mixed feed, pigs were challenged orally with 1011 CFU of ETEC and body weight, diarrhea score, bacterial CFU and phage PFU in the feces were measured. Pigs of phage treated groups C (106 PFU/g) and D (108 PFU/g) showed more resistance to diarrhea due to ETEC infection compared to positive control group B on the third day after the initial challenge. Moreover, during the quantitation of ETEC in feces, both groups C and D showed approximately 63.92 and 60.73% reduced ETEC compared to positive control group B. Phages were successfully isolated from feces in both groups C and D during the experiment without any adverse effects, suggesting the possibility of using CJ12 as a feed additive.
著者
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Shin Seung
Department Of Electrical Engineering Korea Advanced Institute Of Science And Technology
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Cha Seung
Department Of Ceramic Engineering Yonsei University
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Lee Won
Department Of Agricultural And Biological Engineering University Of Florida
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Yoo Han
Department Of Infectious Disease College Of Veterinary Medicine And Krf Zoonotic Disease Priority Re
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Jung Myung
Department Of Biochemistry And Molecular Biology Center For Chronic Metabolic Disease Research Colle
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Shin Min
Department Of Psychiatry Seoul National University College Of Medicine
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YOO An
Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute, Brain Korea 21 for Veterinary Science, Seoul National University, Seoul 151–742, Korea
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CHO Young
CJ Research Institute of Biotechnology, CJ CheilJedang Co., Seoul 157–724, Korea
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SHIN Seung
Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute, Brain Korea 21 for Veterinary Science, Seoul National University
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SHIN Min
Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute, Brain Korea 21 for Veterinary Science, Seoul National University
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CHO Young
CJ Research Institute of Biotechnology, CJ CheilJedang Co.
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YOO Han
Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute, Brain Korea 21 for Veterinary Science, Seoul National University
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LEE Won
Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute, Brain Korea 21 for Veterinary Science, Seoul National University
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Shin Min
Department of Applied Science, Korea Maritime University, Busan 606-791, Korea
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CHA Seung
Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute, Brain Korea 21 for Veterinary Science, Seoul National University
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JUNG Myung
Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute, Brain Korea 21 for Veterinary Science, Seoul National University
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YOO An
Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Disease Priority Research Institute, Brain Korea 21 for Veterinary Science, Seoul National University
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