流産が発生した養豚場から分離した細胞変性作用を示すMycoplasma hyorhinisの性状
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概要
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流産が頻発する養豚場から分離されたウイルス様の細胞変性作用を示す病原体について検討した.その細胞変性作用を示す病原体の遺伝子情報を得るためディファレンシャルディスプレイRT-PCR (ddRT-PCR)を用いた.ディファレンシャルディスプレイPCRで得られた527塩基の部分的DNA塩基配列はMycoplasuma hyopneumoniaeの23SrRNA遺伝子と88.7%の相同性を示した.さらに意外にも5'末端の333塩基配列はヒトのガン遺伝子PTI-Iの5'の非翻訳領域と96.1%の相同性を示した.培地にBM-cyclinを加えると細胞変性作用と蛍光染色法で核外DNAの蛍光が観察されなくなったことから, BM-cyclinに感受性のマイコプラズマ様病原体が細胞変性を引き起こしていることが推察された.さらに,トリチウムチミジンとトリチウムウリジンを取り込ませた病原体の浮遊密度が1.20-1.24 g/dlで単一ピークを示し,病原体の超微構造からもこの細胞変性を示す病原体がマイコプラズマであることが示唆された.その病原体はM. hyopneumoniaeが増殖可能なFriis培地で増殖できなかった.細胞変性を示す病原体から得られた16SrRNAのDNA塩基配列は複数のM. hyorhinis菌株とはO.8-1.0%の塩基配列の相違が認められ,新たに分離されたマイコプラズマ様病原体はM. hyorhinisの変異株であることが示唆された. M. hyorhinisの23SrRNA遺伝子の一部とヒトのガン遺伝子PTI-Iの5'の非翻訳領域との高い相同性はM. hyorhinisがヒトのガン遺伝子PTI-Iの進化と.関連性があることを示唆している.
- 2003-04-25
著者
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SHIN Jin
Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of
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Lee Won
Department of Internal Medicine, institute of Medical Research, Sungkyunkwan University School of Me
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Joo Han
Department Of Clinical And Population Sciences College Of Veterinary Medicine
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Lee Won
Department Of Clinical And Population Sciences College Of Veterinary Medicine
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SEOK H0
Department of Clinical and Population Sciences,College of Veterinary Medicine
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CALSAMIG Maria
Department of Clinical and Population Sciences,College of Veterinary Medicine
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PIJOAN Carlos
Department of Clinical and Population Sciences,College of Veterinary Medicine
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W. MOLITOR
Department of Clinical and Population Sciences,College of Veterinary Medicine
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Lee Won
Department Of Agricultural And Biological Engineering University Of Florida
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Seok H0
Department Of Clinical And Population Sciences College Of Veterinary Medicine
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Shin Jin
Department Of Anesthesiology And Pain Medicine Asan Medical Center University Of Ulsan College Of Me
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W. Molitor
Department Of Clinical And Population Sciences College Of Veterinary Medicine
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Shin Jin
Department Of Clinical And Population Sciences College Of Veterinary Medicine
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Pijoan C
Department Of Clinical And Population Sciences College Of Veterinary Medicine
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Calsamig Maria
Department Of Clinical And Population Sciences College Of Veterinary Medicine
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Pijoan Carlos
Department Of Clinical And Population Sciences College Of Veterinary Medicine
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