Pressurized liquid method for fucoxanthin extraction from Eisenia bicyclis (Kjellman) Setchell(METHODS)
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概要
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Optimization of extraction conditions for fucoxanthin from brown algae Eisenia bicyclis was investigated through a pressurized liquid extraction (PLE) method and statistical experimental design. The process was optimized by Plackett-Burman design at first step to screen the most important variables in the extraction of fucoxanthin, and subsequently central composite design was applied to attain the optimum conditions of the selected factors for fucoxanthin extraction. Two factors, temperature and ethanol concentration, significantly influenced the extraction efficiency of fucoxanthin at 95% level (P<0.05). The maximum predicted value of fucoxanthin extraction was O.42mg/g at 110℃ and 90% ethanol. The validation of the model was verified by triplicate experiments under the optimal conditions. The results demonstrated that the statistical strategy was successfully applied for optimization of PLE method for fucoxanthin extraction and that PLE can be a powerful method to extract fucoxanthin from E. bicyclis.
著者
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Lee Won
Department of Internal Medicine, institute of Medical Research, Sungkyunkwan University School of Me
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Lee Won
Department Of Agricultural And Biological Engineering University Of Florida
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Um Byung-hun
Natural Product Research Center Kist Gangneung Institute Techno Valley
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Lee Won
Department Of Food Science Gangneung-wonju National University
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Shang Ya
Natural Product Research Center, KIST Gangneung Institute, Techno Valley
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Kim Sang
Natural Product Research Center, KIST Gangneung Institute, Techno Valley
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Shang Ya
Natural Product Research Center Kist Gangneung Institute Techno Valley:department Of Food Science Ga
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Kim Sang
Natural Product Research Center Kist Gangneung Institute Techno Valley
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Shang Ya
Natural Product Research Center Kist Gangneung Institute
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