実験的Fanconi症候群家兎腎刷子縁膜小胞におけるNa+/H+ antiport

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Na+/H+ antiport was investigated in renal brush border membrane vesicles prepared from control and anhydro-4-epitetracycline-administered (100 mg/kg body weight) rabbits. Proton transport and resulting pH-gradient (ΔpH) across membrane were measured using acridine orange as a fluorescent probe. Our system to evaluate Na+/H+ antiport appeared to be consistent with previous descriptions on Na+/H+ antiporter in renal brush border membrane vesicles. We employed pH jump studies to measure initial ratesof H+ transport as a function of Na+ concentration. Kinetic analyses indicate that apparent KNa or Vmax in vesicles from anhydro-4-epitetracycline-administered rabbits was not significantly different from that in control (KNa=4.3±0.4 vs. 5.6±2.2 mM, Vmax=0.161±0.44 vs. 0.194±0.023 pH units/3s, respectively). These results support the conclusion that anhydro-4-epitetracycline dose not affect renal Na+/H+ antiporter.
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