実験的非定型抗酸菌症に関する研究1. 非定型抗酸菌のマウスに対する病原性について (i) : 経尾静脈感染の成績

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Relatively high dose of various atypical mycobacteria were inoculated intravenously into conventional mice (dd strain) and the pathological processes were evaluated mainly by the successively recovered viable units of bacilli in the lungs, spleens and kidneys. In addition, the mean body weights of mice, macroscopic pathological findings as well as root indices of lung and spleen were used for the comparisons of the pathogenicities of the various atypical mycobacteria. One strain of M. kansasii, one of M. scrofulaceum, 5 of M. intracellulare, one of M. xenopi and one of M. fortuitum as well as M. tuberculosis (Kurono) as for the comparison were injected to each group of 20 mice. The inoculum sizes were in the range of 2.0×105 to 1.4×108 viable units of bacilli. Three mice of each group were sacrificed at 24 hours, 3 days, 9 days, 16 days, 30 days and 44 days after inoculation. The organs (lung, spleen and kidney) were emulsified by Teflon homogenizers adding the suitable amount of 2% NaOH aqueous solution and were cultured on 1% Ogawa media by successive ten times-dilutions in order to calculate the viable units of bacilli in each organ. At the same time, the weights of the organs and the macroscopic patho logical findings were evaluated. The remaining mice were sacrificed for the microscopic histopathological sections at the end of the observation. No mouse died in this period of observation except two, one infected with M. tuberculosis, which showed an extensive pulmonary lesions at autopsy and another, which was infected with M. intracellulare and showed no macroscopic pathology. Normal increase of body weights were moderately suppressed in the mice infected with M. tuberculosis (Kurono) and M. kansasii, however, remaining groups of mice infected with various atypical mycobacteria other than M. kansasii showed apparently normal gains of their body weights. On the observation of macroscopic pathology, the pathologic lesions provoked by various atypical mycobacteria were as a whole minimal to moderate, if any, and not constant in the lungs as well as in other organs, though extensively far advanced lesions were detected constantly in the lungs of mice infected with M. tuberculosis (Kurono) towards 16 days after inoculation. One exception was the lesions in the kidneys provoked by M. fortuitum (No.49), which were rather constantly confirmed in the latter half of the observation with moderate severity. The results of the calculations of the culturable acid-fast bacilli as well as the microscopic findings suggested a lower pathogenicity of mycobacteria other than human tubercle bacilli. Although the culturable bacilli in the lungs showed a tendency of steady increase in the mice infected with M. tuberculosis, apparent spontaneous regressions of the pulmonary lesions in the mice infected with various atypical mycobacteria were suggested by frequent decreases of culturable bacilli towards the end of the observation, and occasional rapid disappearances of the culturable bacilli confirmed again a definite low pathogenicity of atypical mycobacteria for mice.
日本結核病学会の論文

実験的非定型抗酸菌症に関する研究1. 非定型抗酸菌のマウスに対する病原性について (i) : 経尾静脈感染の成績

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