A Basic Study on Clinical Application of High Magnification Fiber-Gastroscope I:I Observation of Gastric Surface and Gastric Spirillum by Scanning Electron Microscope

概要

論文の詳細を見る
Though the diagnosis of early gastric cancer has recently been made more and more accurate, there are still difficulties remaining in the recognition of small and early malignant changes or in differential diagnosis of some atypical morbid conditions from other common gastric diseases. In order to remove these difficulties, a highly magnificated fiber-gastroscope has recently been developed, but it is frequently disturbed by mucous covering the gastric surface. We tried to clean the mucous by Gascon drop and the cleaning efficacy was checked by scanning electron microscope. We also observed gastric spirillum appearing on the gastric surface by scanning electron microscope.Materials and Methods:Two rhesus monkeys were used, whose stomachs were immediately removed and washed in 150ml of 10% Gascon drop-water solution, once in one monkey and twice in the other. The specimens were taken at corpus and antrum, fixed with 2.5% buffered glutaraldehyde (pH7.5) and then dehydrated with acetone and coated with gold about 100A thick. They were examined with the scanning electron microscope, type JSM-U3.Results:Gastric area, sulcus, and the surface of the surface mucous cells were successfully observed and photos were taken clearly in the specimen washed twice, but not in the specimen washed only once. In the cleansed surface, the large number of microvilli of 400 or 500A in diameter were recognized all over surface mucous cells.In the specimen washed only once, these characteristic features were not observed so distinctly, while microspirals sized 60μ in length and 0.95μ in diameter were clearly observed in them.

A Basic Study on Clinical Application of High Magnification Fiber-Gastroscope I:I Observation of Gastric Surface and Gastric Spirillum by Scanning Electron Microscope

スポンサーリンク

概要

著者

関連論文

スポンサーリンク