The Basic Study on the Clinical Application of High Magnification Fiber-Gastroscope:II. Observation of the Epithelial Surface of Human Stomach by Scanning Electron Microscope

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Fine structures of epithelial surface of human stomach removed by surgical operation, were subjected to the scanning electron microscopical observation.The specimens were taken from the scar of a gastric linear ulcer and its surrounding epithelial surface of the gastrectomized materials of a 43 year old man. The specimens were immediately washed with Dimethylpolysiloxane solution, fixed with 2.5% buffered glutaraldehyde and dehydrated with acetone. After coating with gold about 100Å thick, they were subjected to the observation and were photographed by scanning electron microscope, JSM-U3.The gastric surface was uneven, where two types of foveola were observed. Ring-like one was named foveolar type and ladder-like one was named to sulciolar type.In the scar of gastric linear ulcer covered by regenerated epithelial tissues the sulciolar typed of foveola was found on the oral part and the foveolar type was located on the anal part.We were able to observe the surface of the gastric surface of each epithelial cells by ×3000 of the magnification. Gastric epithelial cells were covered with mucous and after the removal of the mucous the surface of epithelial cells was seen with their projections and mucous threads. Microvilli-like structures were also observed, but they are not enough clear. From these studies, we feel the importance to develope further the washing method of the gastric mucous in order to get a more accurate gastroscopic diagnosis.