:3. Comparison with Chang liver cell as a target cell and studies on the surface marker of cytotoxic lymphocyte

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The cytotoxicity of peripheral blood lymphocytes against Chang liver cell and LiC-l (Ohtsuka) was studied by microcytotoxicity assay using separated lymphocyte subpopulations of E rosette forming cells and non E rosette forming cells. Cell surface markers of cytotoxic lymphocytes against these target cells were studied by direct immunofluorescence method.T cell depleted population of the peripheral blood lymphocytes exhibited the strong cytotoxic activity against Chang liver cell in patients with chronic active hepatitis and liver cirrhosis. The lymphocytes from healthy donors showed only slight cytotoxicity against Chang liver cell. The lymphocytes in the process of cytotoxicity against Chang liver cell in vitro showed Fc receptor on cell surface which was detected by FITC labeled human heat aggregated IgG, but not surface Ig by FITC labeled anti-human Ig goat antibody. This effector against Chang liver cell was considered to be mainly "K cell".The cytotoxicity of peripheral blood lymphocytes from healthy donor against Chang liver cell was enhanced by addition of sera from patients with chronic active hepatitis. This cytotoxicity was mediated by the lymphocytes which belonged to T cell depleted population and exhibited Fc receptor on cell surface but not surface Ig. This effector was con- sidered to be mainly "K cell".The statistically significant difference was not observed in the cytotoxicity against LiC-1 between healthy controls and patients with liver cirrhosis or hepatoma. The cytotoxicity against LiC-1 was mediated by the lymphocytes which belonged to T cell depleted population and exhibited Fc receptor on cell surface and some of which exhibited surface Ig also. This effector was considered to be mainly "natural killer cell (NK cell) ".
財団法人日本消化器病学会の論文

:3. Comparison with Chang liver cell as a target cell and studies on the surface marker of cytotoxic lymphocyte

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