Enzymatic Hydrolysis of <SUP>125</SUP>I-Labeled Parathyroid Hormone
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概要
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Release of trichloracetic acid-soluble <SUP>125</SUP>I from <SUP>125</SUP>I-labeled parathyroid hormone was measured after incubation with carrier parathyroid hormone and enzyme preparation obtained from rat kidney with or without inhibitors for 60 minutes at 38°C in order to evaluate parathyroid hormone hydrolyzing or <SUP>125</SUP>I-PTHase activity. More <SUP>125</SUP>I was released as the amount of enzyme preparation was increased, the time of incubation prolonged, or the amount of carrier parathyroid hormone decreased. Maximum <SUP>125</SUP>I releasing activity of rat kidney homogenate was found at pH 8.5, while that of rat kidney supernatant was found at pH 4.5. The former enzyme activity was quite predominant in the kidney, while the latter was also found in the spleen in similar concentration. Rat kidney microsome fraction contained the former most abundantly, while supernatant is the main site of the presence of the latter. The 125 I-releasing activity at pH 8.5 of the EDTA treated rat kidney homogenate was activated by Co<SUP>++</SUP> and Fe<SUP>++</SUP> and that of acetone powder extract of rat kidney by Co<SUP>++</SUP>, Mn<SUP>++</SUP>, Ni<SUP>++</SUP>, and Fe<SUP>++</SUP>. <SUP>125</SUP>I-PTHase activity was definitely inhibited by p-chloromercuribenzoate and mercaptoethanol but scarcely by puromycin, monoiodoacetate, 1-arginine, 1-lysine, tosylphenylalanine chloromethylketone, serotonin, phenylacetate, and 0-phenanthroline.
- 社団法人 日本内分泌学会の論文
著者
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FUJITA Takuo
Calcium Research Institute
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Ohata Masahiro
Department Of Internal Medicine Wakayama Medical College Kihoku Branch Hospital
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Yoshikawa Masaki
Department Of Cardiology Tsuyama Center Hospital
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Orimo Hajime
Department Of Endocrinology Tokyo Metropolitan Geriatric Medical Center
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Fujita Takuo
Department Of Internal Medicine Kobe University School Of Medicine
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MARUYAMA MOTOHIRO
Central Research Laboratories Sankyo Co., Ltd.
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FUJITA TAKUO
Department of Anatomy, Jikei University School of Medicine
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OHATA MASAHIRO
Department of Geriatrics University of Tokyo Faculty of Medicine
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ORIMO HAJIME
Department of Endocrinology and Metabolism, Tokyo Metropolitan Geriatric Hospital
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ORIMO HAJIME
Department of Geriatrics University of Tokyo Faculty of Medicine
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FUJITA TAKUO
Department of Geriatrics University of Tokyo Faculty of Medicine
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