1-34 Human Parathyroid Hormone Radioimmunoassay: Properties of Antiserum Against Synthetic 1-34 Human Parathyroid Hormone and Its Clinical Application
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概要
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Antibody against synthetic 1-34 humanPTH synthesized by Niall <I>et al</I>.(1974), was developed in rabbits. Synthetic 1-34 hPTH was found to be a good immunogen for rabbit. The relative importance of various structural parts of 1-34 hPTH molecule with regard to immunological specificity was determined by reference to inhibition of specific binding of <SUP>125</SUP>I-1-34 hPTH to the antibody by analogues of 1-34 hPTH (NLeu<SUP>8</SUP>-NLeu<SUP>18</SUP> 1-34 hPTH, NLeu<SUP>8</SUP>-NLeu<SUP>18</SUP>-Tyr<SUP>34</SUP>hPTH) and by 1-34 bPTH. The immunological recognition site in 1-34 hPTH molecule was found to be located around the 8 to 18 amino acid sequence, because the binding affinity to the antibody of this analogue (NLeu<SUP>8</SUP>-NLeu<SUP>18</SUP>1-34 hPTH) was less than that of the native hormone in the antibody. Furthermore, other immunological recognition sites were located in the C- and N-terminal regions of this molecule, as reported by Segre <I>et al</I>.(1976) and Visser <I>et al</I>.(1979).<BR>This antiserum could measure only 1-34 hPTH molecule in serum, since it did not crossreact with partially purified 1-84 hPTH.<BR>In order to evaluate the advantage of 1-34 hPTH radio-immunoassay (RIA) in the diagnosis of parathyroid dysfunction, serum PTH levels in various diseases were measured by both 1-34 hPTH RIA and 1-84 PTH RIA and the values obtained by these assays were compared.<BR>There was a good correlation between the values obtained by 1-34 hPTH RIA and those by 1-84 PTH RIA. However, in patients with chronic renal failure, the incidence of cases with high serum PTH level was 90% when measured by 1-84 PTH RIA while it was 39% when measured by 1-34 hPTH RIA. Serum PTH levels in primary hyperparathyroidism were abnormally high and those in hypoparathyroidism were low in both assays. Some patients with senile osteoporosis had a high serum PTH level. The incidences of cases with a high serum PTH level in this disease were equal in both assays.<BR>In conclusion, these two site specific RIAs (1-34 PTH RIA and 1-84 PTH RIA) were useful in the evaluation of PTH secretion and / or metabolism.
- 社団法人 日本内分泌学会の論文
著者
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Orimo Hajime
Department Of Endocrinology Tokyo Metropolitan Geriatric Medical Center
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YAMAUCHI HIROSE
Department of Isotopes, Tokyo Metropolitan Institute of Gerontology
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Shiraki Masataka
Department Of Internal Medicine Research Institute And Practice For Involutional Diseases
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Shiraki M
成人病診療研究所
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KAWADA NORIHIKO
Department of Endocrinology and Metabolism, Tokyo Metropolitan Geriatric Hospital
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AKIGUCHI ITARU
Department of Endocrinology and Metabolism, Tokyo Metropolitan Geriatric Hospital
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KOJIMA ATSUKO
Department of Endocrinology and Metabolism, Tokyo Metropolitan Geriatric Hospital
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ORIMO HAJIME
Department of Endocrinology and Metabolism, Tokyo Metropolitan Geriatric Hospital
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YAMAUCHI HIROSE
Department of Endocrinology and Metabolism, Tokyo Metropolitan Geriatric Hospital
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SHIRAKI MASATAKA
Department of Endocrinology and Metabolism, Tokyo Metropolitan Geriatric Hospital
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