Differential Hydrolysis of Bovine Parathyroid Hormone and its N-terminal Peptide by Rat Kidney
スポンサーリンク
概要
- 論文の詳細を見る
Highly purified bovine parathyroid hormone (b-PTH 1-84) and its synthetic N-terminal peptide (b-PTH 1-34) were labelled with <SUP>125</SUP>I and incubated with rat kidney homogenate at 37°C for 1 hour to assess the degree of hydrolysis of the iodinated peptides through measurement of the increase of trichloroacetic acid soluble 125I fraction. Rat kidney homogenate rapidly hydrolyzed b-PTH 1-84 but was scarcely effective in hydrolyzing b-PTH 1-34. When <SUP>125</SUP>I labelled b-PTH 1-84 and b-PTH 1-34 were injected intravenously in rats, hydrolysis <I>in vivo</I> of the former appeared to be much more rapid than that of the latter, as showh by the faster disappearance from plasma of trichloroacetic acid precipitable fraction. Incubation of b-PTH (1-84) with rat kidney homogenate caused a shift of <SUP>125</SUP>I PTH peak almost to the position of salt peak, while the position of <SUP>125</SUP>I b-PTH (1-34) was almost unchanged by incubation with rat kidney homogenate. N-terminal peptide of bovine parathyroid hormone thus appears to be less susceptible to hydrolytic degradetion by rat tissue than the intact hormone, with resultant longer retention in the blood stream.
- 社団法人 日本内分泌学会の論文
著者
-
Ohata Masahiro
Department Of Internal Medicine Wakayama Medical College Kihoku Branch Hospital
-
Yoshikawa Masaki
Department Of Cardiology Tsuyama Center Hospital
-
Fujita Takuo
Department Of Internal Medicine Kobe University School Of Medicine
-
OKANO KAZUTOSHI
Department of Geriatrics, University of Tokyo Faculty of Medicine
関連論文
- PE-579 Inhibitory effects of Pioglitazone on Migration, Proliferation and Reactive Oxygen Species Production in Human Coronary Artery Smooth Muscle Cells(Restenosis basic/clinical(03)(IHD),Poster Session(English),The 72nd Annual Scientific Meeting of the
- A-32. Dynamic Study on Cerebrospinal Fluid Circulation After Subarachnoid Hemorrhage : Second report
- S-II-7. Studies on Cerebrospinal Fluid Circulation Following Subarachnoid Hemorrhage
- Effects of menatetrenone on the bone and calcium metabolism in osteoporosis: A double-blind placebo-controlled study
- Biclonal Gammopathy Associated with Rectal Cancer and Duodenal Ulcer
- PJ-513 Relationship between Serum Oxidized Low-Density Lipoprotein and Other Risk Factors for Atherosclerosis and Metabolic Syndrome(Lipid disorders-3, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-351 Effects of Combined Treatment with Statin and Angiotensin Receptor Blocker on Stent Restenosis and Human Coronary Artery Smooth Muscle Cells(Restenosis basic/clinical-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Effect of Acetic Acid or Rice Vinegar Feeding after Exercise on Blood Glucose in Fasted and Running-trained Rats
- CORONARY ATHEROSCLEROSIS IN THE CASES OF SUDDEN CARDIAC DEATH, WITH SPECIAL REFERENCE TO THE CARDIAC RUPTURE : PROCEEDINGS OF THE 38TH ANNUAL MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Changes of serum calcitonin in stress load
- Hypoxemia is a risk factor for bone mass loss
- Areal bone mineral density and bone phosphatase activities in experimental diabetes mellitus
- PLATELET AGGREGATION IN ISCHEMIC HEART DISEASE
- Skin Amyloid Deposition in Systemic Amyloidosis in a Patient with IgD M-protein
- EFFECT OF SMOKING ON PLATELET AGGREGATION AND FIBRINOLYSIS IN ISCHEMIC HEART DISEASE : Ist Auditorium
- Ultimobranchial calcitonin of the eel, Anguilla japonica
- Effect of Calcitonin on the Development of Immobilization Osteoporosis in Rat
- Pyrophosphate Enhancement of Hypocalcemic Effect of Thyrocalcitonin in Rat
- Pyrophosphate Enhancement of Thyrocalcitonin Effect in Tissue Culture
- ENDOCRINE EFFECTS ON PLASMA CALCIUM IN PARATHYROIDECTOMIZED RATS
- In vivo Effect of Parathyroid Hormone and/or Calcitonin on the Lysosomal Enzyme Activity of Rat Bone
- Enzymatic Hydrolysis of 125I-Labeled Parathyroid Hormone
- EFFECT OF AGE AND THYROCALCITONIN ON MYOCARDIAL CHANGES INDUCED BY SODIUM SULFAACETYLTHIAZOLE
- PREVENTION OF EXPERIMENTAL BONE ATROPHY BY THYROCALCITONIN
- A TEST ON THE RECOVERY FROM HYPERCALCEMIA FOR EVALUATION OF THYROCALCITONIN ACTIVITY IN MAN
- SERUM CALCIUM RESPONSE TO DISODIUM ETHYLENDIAMINE-TETRAACETATE (Ca-Na2-EDTA) AS A SIMPLE RAPID DIAGNOSTIC TEST FOR HYPOPARATHYROIDISM
- Parathyroid Hormono-hydrolyzing Enzymes in Human Kidney
- Effects of Exercise Load on Hepatic Blood Flow in Patients with Chronic Liver Diseases
- Effect of Parathyroidectomy on Bone Atrophy Induced by Renal Injury with Na-Sulfacetylthiazole
- Effect of Renal Injury on the Activity of Enzymes Preferentially Hydrolyzing Parathyroid Hormone
- A Case of Pseudohypoparathyroidism with Increased Bone Turnover and Demineralization
- Effects of 1α-Hydroxyvitamin D3 on Experimental Uremic Renal Osteodystrophy in Rats Induced by Na-Sulfacetylthiazole
- Effects of Age and Diseases on Human Serum 25-Hydroxycholecalcif erol Determined by Competitive Protein-Binding Assay
- Endocrine Factors in Senile Osteoporosis
- Physiological Significance of an Enzyme Preferentially Hydrolyzing Parathyroid Hormone in Rat Kidney
- Crystals in Hyperfunctioning Rat Parathyroid Cells
- Endocrine Control of the Intestinal Calcium Excretion
- A Radioimmunoassay for Rat Serum Parathyroid Hormone using N-terminal of PTH
- Differential Hydrolysis of Bovine Parathyroid Hormone and its N-terminal Peptide by Rat Kidney
- Radioimmunoassay of Serum Parathyroid Hormone in Postmenopausal Osteoporosis
- THE TIMING OF THE KOROTKOFF SOUNDS: A MEASURE OF HYPERTHYROIDISM
- Intermittent arm ischemia induces vasodilatation of the contralateral upper limb
- A Case of Renal Tubular Acidosis with Secondary Hypherparathyroidism, Lipemia and Aminoaciduria with Review of Japanese Literature