Effects of Age and Diseases on Human Serum 25-Hydroxycholecalcif erol Determined by Competitive Protein-Binding Assay
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概要
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Competitive protein-binding assay of 25-OH-D was developed by the use of specific vitamin D-binding proteins from vitamin D-deficient rat serum. Ether extract of serum sample which was dried and dissolved in ethanol, or standard solution of 25-OH-D3, was incubated with <SUP>3</SUP>H-25-OH-D<SUB>3</SUB> and vitamin D-binding protein for 2 hours at 4°C. Free and bound <SUP>3</SUP>H-25-OH-D<SUB>3</SUB> were separated through dextran-coated charcoal. The sensitivity of the assay system was 0.22 ng/tube. Percent cross reaction in the assay was 2.18% in vitamin D<SUB>3</SUB>, 0.70% in 1, 25-(OH) <SUB>2</SUB>-D<SUB>3</SUB>, less than 0.28% in la-OH-D<SUB>3</SUB>, and less than 0.06% in dihydrotachysterol, cholesterol and cortisol.<BR>Human serum 25-OH-D is 28.9±2.9 ng/m<I>l</I> in 19 normal subjects. Serum 25-OH-D in the old-age group (50-70 years of age) was significantly decreased, compared with that in the young-age group (20-40 years of age). Serum 25-OH-D was significantly decreased in gastrectomized and osteoporotic patients as well as in the patients with liver cirrhosis, in comparison with their age-controls.
- 社団法人 日本内分泌学会の論文
著者
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Yoshikawa Masaki
Department Of Cardiology Tsuyama Center Hospital
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Goto Hajime
Department Of Particle And Nuclear Physics Graduate University For Advanced Studies
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OKANO KAZUTOSHI
Department of Geriatrics, University of Tokyo Faculty of Medicine
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NAKAI RUMIKO
Department of Geriatrics, Faculty of Medicine, University of Tokyo
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GOTO HAJIME
Department of Geriatrics, University of Tokyo Faculty of Medicine
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NAKAI RUMIKO
Department of Geriatrics, University of Tokyo Faculty of Medicine
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