The Effect of Ovarian Steroid Feedback upon Radioimmunoreactive Luteinizing Hormone Releasing Hormone in the Hypothalamus
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概要
- 論文の詳細を見る
A radioimmunoassay (RIA) method for luteinizing hormone (LH) releasing hormone (RH) utilizing rabbit antiserum against synthetic (Glu<SUP>1</SUP>)-LH-RH coupled with human serum albumin at the N-terminus, is described. This assay system for LH-RH also cross-reacted with several LH-RH analogues or fragments, but not with pituitary trophic hormones. The assay was performed on the hypothalamic extracts of adult ovariectomized rats and female immature rats which had been treated with estradiol. The FSH and LH levels in the pituitary gland and serum of the same animals were determined by RIA. The radioimmunoreactive LH-RH content of the stalk median eminence markedly increased seven days after ovariectomy. The serum levels and the pituitary contents of FSH and LH of the same rats were also significantly augmented. In immature rats, the hypothalamic content of LH-RH, as measured by RIA, was significantly increased one hour after the injection of estradiol. The FSH and LH levels in the pituitary showed a significant rise after 7 hours.
- 社団法人 日本内分泌学会の論文
著者
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Okinaga Shoichi
Department of Obstetrics & Gynecology, Teikyou University, School of Medicine, Ichihara Hospital,
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Yanaihara Takumi
Department Of Obstetrics And Gynecology School Of Medicine Showa University
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Arai Kiyoshi
Department Of Pediatric Dentistry Nihon University Graduate School Of Dentistry At Matsudo
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Yanaihara Chizuko
Department Of Bioorganic Chemistry Shizuoka College Of Pharmacy
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Yanaihara Noboru
Department Of Anatomy Hiroshima University School Of Medicine:laboratory Of Bioorganic Chemistry Shi
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KANAZAWA MOTOMI
Department of Obstetrics and Gynecology, Teikyo University School of Medicine
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Arai Kiyoshi
Department Of Obstetrics And Gynecology Teikyo University School Of Medicine
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YANAIHARA NOBORU
Department of Obstetrics and Gynecology, Teikyo University School of Medicine
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YANAIHARA CHIZUKO
Department of Bio-organic Chemistry, Shizuoka College of Pharmacy
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YANAIHARA CHIZUKO
Department of Obstetrics and Gynecology, Teikyo University School of Medicine
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YANAIHARA TAKUMI
Department of Obstetrics and Gynecology, Teikyo University School of Medicine
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