Radioimmunoassay of Human Proinsulin C-peptide Using Synthetic Human Connecting Peptide

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概要

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• Radioimmunoassay of human C-peptide was established using antiserum to synthetic human connecting peptide and synthetic tyrosylated human connecting peptide. Synthetic human connecting peptide and natural human C-peptide showed the same degree of cross-reactivity with the synthetic human connecting peptide antiserum. Natural human proinsulin reacted approximately one fourth as well as the human connecting peptide or natural human C-peptide when expressed on an equimolar basis.<BR>Synthetic porcine and bovine connecting peptide, human insulin and natural porcine proinsulin did not react with the human connecting peptide antiserum. Synthetic human connecting peptide did not cross with insulin antiserum. The senstitivity of the radioimmunoassay was 0.05ng/tube.<BR>The assay system was available to determine C-peptide immunoreactivity in plasma, because proinsulin and proinsulin-like components had been reported to be very low in plasma. The fasting level of C-peptide immunoreactivity in plasma of normal subjects assayed by this system was 0.88±0.21ng/m<I>l</I>.

• 社団法人 日本内分泌学会の論文

著者

• Oda Toshitsugu

  First Department Of Internal Medicine Faculty Of Medicine University Of Tokyo

• Oka Hiroshi

  First Department Of Internal Medicine Tokyo University

• Yanaihara Chizuko

  Department Of Bioorganic Chemistry Shizuoka College Of Pharmacy

• KANEKO TOSHIO

  First Department of Internal Medicine, Faculty of Medicine, University of Tokyo

• Suzuki Seiji

  First Department Of Internal Medicine Showa University Fujigaoka Hospital

• YAMASHITA KAMEJIRO

  First Department of Medicine, University of Tokyo School of Medicine

• YANAIHARA NOBORU

  First Department of Internal Medicine, University of Tokyo Faculty of Medicine

• MUNEMURA MASAHIDE

  First Department of Internal Medicine, University of Tokyo Faculty of Medicine

• HASHIMOTO TADASHI

  First Department of Internal Medicine, University of Tokyo Faculty of Medicine

• Oka Hiroshi

  First Department Of Internal Medicine Faculty Of Medicine University Of Tokyo

• YANAIHARA CHIZUKO

  Department of Bio-organic Chemistry, Shizuoka College of Pharmacy

• KANEKO TOSHIO

  First Department of Internal Medicine, University of Tokyo Faculty of Medicine

• YAMASHITA KAMEJIRO

  First Department of Internal Medicine, University of Tokyo Faculty of Medicine

• SUZUKI SEIJI

  First Department of Internal Medicine, University of Tokyo Faculty of Medicine

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