BIOSYNTHESIS OF STEROIDS BY HUMAN FETAL ADRENAL PERFUSED IN VITRO

概要

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Formation of steroids in fetal tissue has been studied mostly by incubation technique. In the present experiment, human fetal adrenal obtained from two fetuses aborted at midtrimester were perfused in vitro with labeled precursors including ^3H-pregnenolone-sulfate and ^<14>C-dehydroepiandrosterone (DHA). Both adrenal and kidney wer perfused at the same time in this study. As perfusate, 100 ml of oxygenated type O blood diluted with saline was used. Perfusion was performed without recirculation. Radioactive metabolites extracted after the termination of perfusion from the perfusate and the tissue were hydrolysed, separated and isolated by Hyflo-Supercel partition column chromatography followed by thin layer chromatography. Radiochemical purity of the metabolites was achieved by recrystallization to constant specific activity. Perfusion of the fetal adrenal resulted in the formation of both labeled DHA and 16_αOH-DHA, tritiated 16_αOH-pregnenolone and 17_αOH-pregnenolone. Since those metabolites were mainly found in the perfusate, it is suggested that the steroids formed in the tissue were easily released to the circulatory system. Formation of 16_α-hydroxylated steroids in the fetal adrenal and the role of those compounds on the formation of estriol during pregnancy are discussed.
社団法人日本産科婦人科学会の論文
1977-11-01