円形薄層クロマトグラフィーによるアミノ酸類の分離

概要

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A new type of apparatus for the circular thinlayer chromatography in which the developing solvent is fed by dropping method has been constructed and applied for the separation and identification of amino acids. Circular chromatoplates of Silica Gel G and Aluminium Oxide G, 0.2 mm thick and 200 mm in diameter, were employed. The sample solution of mixed amino acids was placed at the center or on the circumference around the center with a 10 mm radius on a circular chromato-plate and a developing solvent was fed onto the center of this plate. Phenol-water (75:25, w/w) and n-butanol-glacial acetic acid-water (40:10:50, v/v) were used for Silica Gel G plate and Aluminium Oxide G plate respectively, and ninhydrin was used as a visualization reagent. The resulting chromatogram showed well defined concentric rings or arcs of amino acids. The amino acids present in the natural products could be separated and identified within a distance of about 90 mm past the sample origin. The time required for the development was about 180 minutes.
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