海産浮遊珪藻Skeletonema costatum (GREV.) CLEVEの寒天培地でのコロニー形成

概要

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Marine planktonic diatom Skeletonema costatum (GREV.) CLEVE is a notorious phytoplankton which often caused red tide in the coastal waters anywhere. S. costatum was found to grow and form in/on an agar plate in this study whereas almost centric diatoms had been scarcely grown on the solidified medium.S. costatum used were field samples from Dokai Bay, Hibiki Nada, and Suho Nada, Northern Kyushu, and several clonal strains of uni-algal cultures and axenic cultures from these marine regions. The culture of this species was mainly done by mixed plate method with the agar medium containing various nutrients under the conditions of 1.0-8.0Klx for 14h per day and about 25°C.Most strains of S. costatum used grew well and formed brown colonies in/on an agar plate.These colonies were observable in a visual manner within eight days of incubation. Colonies had disk-like on an agar plate but irregular shapes in it. The cells resuspended into the liquid medium from the colonies in/on agar plates grew as well as the cultures before inoculating into the agar medium. Several strains of S. costatum of axenic cultures grew better in/on agar plates with trypticase than in/on these without it.
公益社団法人日本水産学会の論文