Appearance of reverse transcriptase activity in human fetal thymus cells stimulated by human B-cell.
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概要
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We have investigated three human fetal thymuses by various induction procedures in a search for retrovirus. Thymus cells were cultured for 7 days together with mitomycin C-treated B cells and examined for reverse transcriptase activity. The following results were obtained. 1) RNA-directed DNA polymerase (RDDP) activity was detected in the culture medium on day 3 and persisted until day 7. This enzyme, after partial purification on phosphocellulose column, showed template-primer specificity of a typical reverse transcriptase. 2) In unstimulated thymus cells, no reverse transcriptase activity was detected. 3) Stimulation with lymphocyte mitogens such as phytohaemagglutinin (PHA) and concanavalin A (Con A) exerted no effect on inducing the reverse transcriptase activity. 4) No reverse transcriptase activity was obtained in the culture medium of B cells pretreated with mitomycin C. Taken together these results indicate that in human fetal thymus cells reverse transcriptase activity is induced by human B cell stimulation, pointing to an intriguing possibility of protovirus activation.
- The Keio Journal of Medicineの論文
著者
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SAITO Hidetsugu
Department of Internal Medicine, School of Medicine, Keio University
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Saito Hidetsugu
Department Of Internal Medicine Division Of Gastroenterology Keio University School Of Medicine
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Higashinakagawa Toru
Department Of Biology School Of Education Waseda University
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Tsuchiya Masaharu
Department Of Internal Medicine Keio University
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Nakamura Tatsuya
Department Of Anatomy Kitasato University School Of Medicine
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Ono Akira
Department of 2nd Internal Medicine, Faculty of Medicine, Oaka University
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YOSHIMATSU HIROSHI
Department of Internal Medicine and Surgery, University of Occupational and Environmental Health
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YOSHIMATSU HIROSHI
Department of Surgery, University of Occupational and Environmental Health
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HIGASHINAKAGAWA TORU
Department of Biology, Tokyo Metropolitan University
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KONDO SHUNZO
Department of Developmental Biology, Misubishi-Kasei Institute of Life Science
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