Cloning of the Genes for the Pituitary Glycoprotein Hormone α and Follicle-Stimulating Hormone β Subunits in the Japanese Crested Ibis, Nipponia nippon(Endocrinology)
スポンサーリンク
概要
- 論文の詳細を見る
We have isolated a part of the gene for the pituitary glycoprotein hormone common α subunit (PGHα) and the whole gene for the follicle-stimulating hormone β subunit (FSHβ) in the Japanese crested ibis (Nipponia nippon), a critically endangered bird species in East Asia. The nucleotide sequence of a part of the PGHα gene (5026bp) contained three exons holding the whole coding and 3' untranslated regions, but lacked a 5' untranslated region. Its exon-intron structure was similar to that in mammals, but different from that in teleosts in the location of the second intron. For the FSHβ gene, the nucleotide sequence of 7633bp was assembled from two phage clones. The exon-intron structure of three exons and two introns was similar to that observed in mammals and teleosts. In the putative promoter region of the ibis FSHβ gene, a progesterone responsive element (PRE)-like sequence and two AP-1 responsive element-like sequences reported in the ovine FSHβ gene were not conserved in complete form. The increased number of ATTTA motifs in the putative 3' untranslated region in comparison with those in Japanese quail and chicken FSHβ cDNA suggested that more rapid degradation of FSHβ mRNA occurs in this species. Deduced amino acid sequences of the ibis PGHα and FSHβ showed high similarities with those of the corresponding subunits of other avian species. This is the first report on the genomic sequences of the PGHα and FSHβ in an avian species.
- 社団法人日本動物学会の論文
- 2003-04-25
著者
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Higashinakagawa Toru
Department of Biology, School of Education, Waseda University
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Kawasaki Daisuke
Department of Second Cardiology, National Hospital Organazation Kagoshima Medical Center
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Kawasaki Daisuke
Department Of Biology School Of Education Waseda University
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AOTSUKA Tadashi
Department of Biology, Tokyo Metropolitan University
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Higashinakagawa Toru
Department Of Biology School Of Education Waseda University
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Ishii S
Department Of Biological Science Graduate School Of Science Tokyo Metropolitan University
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Ishii Susumu
Department Of Biology School Of Education Waseda University
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Higashinakagawa T
Department Of Biology School Of Education Waseda University
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Aotsuka Tadashi
Department Of Biological Science Graduate School Of Sciences Tokyo Metropolitan University
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Ishii Susumu
Department of Biological Science, Graduate School of Science, Tokyo Metropolitan University
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